Explore Workflows
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Cell Ranger Build Reference Indices
Cell Ranger Build Reference Indices =================================== |
![]() Path: workflows/cellranger-mkref.cwl Branch/Commit ID: 581156366f91861bd4dbb5bcb59f67d468b32af3 |
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EMG assembly for paired end Illumina
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![]() Path: workflows/emg-assembly.cwl Branch/Commit ID: 25129f55226dee595ef941edc24d3c44414e0523 |
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EMG assembly for paired end Illumina
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![]() Path: workflows/emg-assembly.cwl Branch/Commit ID: 1b0851e6456ccb1fca237a805ff85c53bc9d58c9 |
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EMG QC workflow, (paired end version). Benchmarking with MG-RAST expt.
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![]() Path: workflows/emg-qc-paired.cwl Branch/Commit ID: 583307878ab83c5845c897f03db920ae8e1929e2 |
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Running cellranger count and lineage inference
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![]() Path: definitions/subworkflows/single_cell_rnaseq.cwl Branch/Commit ID: 6b365b79675b2aabfb8d5829bb8df0a6e986b037 |
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Trim Galore RNA-Seq pipeline single-read
The original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for a **single-end** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the single-end RNA-Seq data. It performs the following steps: 1. Trim adapters from input FASTQ file 2. Use STAR to align reads from input FASTQ file according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 3. Use fastx_quality_stats to analyze input FASTQ file and generate quality statistics file 4. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ file to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
![]() Path: workflows/trim-rnaseq-se.cwl Branch/Commit ID: 9e3c3e65c19873cd1ed3cf7cc3b94ebc75ae0cc5 |
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BAM to BEDPE
Comvert BAM to BEDPE and compress the output |
![]() Path: workflows/File-formats/bamtobedpe-gzip.cwl Branch/Commit ID: 527251ebb77750d02dcc9a370d978a153fc9328f |
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kmer_ref_compare_wnode
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![]() Path: task_types/tt_kmer_ref_compare_wnode.cwl Branch/Commit ID: 4b73bfeb967ee9f57a0410276f7c39e784f0846f |
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maf2vcf_gz_workflow.cwl
Workflow to convert a maf file into a vcf.gz with .tbi index |
![]() Path: cwl/maf2vcf_gz_workflow.cwl Branch/Commit ID: ba3ff09328cc646d7254b2d2ee0fbe1abca3d4ad |
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Trim Galore RNA-Seq pipeline single-read strand specific
Note: should be updated The original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for a **single-end** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the single-end RNA-Seq data. It performs the following steps: 1. Trim adapters from input FASTQ file 2. Use STAR to align reads from input FASTQ file according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 3. Use fastx_quality_stats to analyze input FASTQ file and generate quality statistics file 4. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ file to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
![]() Path: workflows/trim-rnaseq-se-dutp.cwl Branch/Commit ID: 8a92669a566589d80fde9d151054ffc220ed4ddd |