Explore Workflows
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Graph | Name | Retrieved From | View |
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Trim Galore RNA-Seq pipeline single-read strand specific
Note: should be updated The original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for a **single-end** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the single-end RNA-Seq data. It performs the following steps: 1. Trim adapters from input FASTQ file 2. Use STAR to align reads from input FASTQ file according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 3. Use fastx_quality_stats to analyze input FASTQ file and generate quality statistics file 4. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ file to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
![]() Path: workflows/trim-rnaseq-se-dutp.cwl Branch/Commit ID: 93b844a80f4008cc973ea9b5efedaff32a343895 |
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SetTelescopeEfficiency
Derive (or set) an overall efficiency parameter to the telescope throughput due to variations of the optical throughput. Allow for dependencies on off-axis angle and wavelength. |
![]() Path: workflows/SetTelescopeEfficiency.cwl Branch/Commit ID: 789752af87eb190387ff2acb4c95c7a5cdb961e7 |
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Cell Ranger Aggregate (RNA+ATAC)
Cell Ranger Aggregate (RNA+ATAC) Combines outputs from multiple runs of “Cell Ranger Count (RNA+ATAC)” pipeline. The results of this workflow are primarily used in “Single-Cell Multiome ATAC and RNA-Seq Filtering Analysis” pipeline. |
![]() Path: workflows/cellranger-arc-aggr.cwl Branch/Commit ID: 93b844a80f4008cc973ea9b5efedaff32a343895 |
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Non-Coding Bacterial Genes
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![]() Path: bacterial_noncoding/wf_bacterial_noncoding.cwl Branch/Commit ID: 424a01693259a75641dc249d553235aa38a6ce23 |
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Trim Galore RNA-Seq pipeline paired-end strand specific
Modified original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for a **pair-end** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the single-end RNA-Seq data. It performs the following steps: 1. Trim adapters from input FASTQ files 2. Use STAR to align reads from input FASTQ files according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 3. Use fastx_quality_stats to analyze input FASTQ files and generate quality statistics files 4. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ files to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
![]() Path: workflows/trim-rnaseq-pe-dutp.cwl Branch/Commit ID: 93b844a80f4008cc973ea9b5efedaff32a343895 |
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umi molecular alignment workflow
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![]() Path: definitions/subworkflows/molecular_alignment.cwl Branch/Commit ID: 4bc0a4577d626b65a4b44683e5a1ab2f7d7faf4c |
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tt_kmer_compare_wnode
Pairwise comparison |
![]() Path: task_types/tt_kmer_compare_wnode.cwl Branch/Commit ID: cd97086739ae5988bab09b05e9259675c4b6bce6 |
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SetLightGuideEfficiency
Set light guide efficiency as function of wavelength and incident angle. |
![]() Path: workflows/SetLightGuideEfficiency.cwl Branch/Commit ID: 789752af87eb190387ff2acb4c95c7a5cdb961e7 |
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kf_STAR_Solo_10x_alignment_wf.cwl
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![]() Path: workflows/kf_STAR_Solo_10x_alignment_wf.cwl Branch/Commit ID: 8c1d2315ad46fe0a30bf618e108e55b0263c7c6d |
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workflow_input_sf_expr_array.cwl
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![]() Path: testdata/workflow_input_sf_expr_array.cwl Branch/Commit ID: b76b039edb62dea76c43f173848cdc57e4b4aab7 |