Workflow: Bacterial Annotation, pass 2, blastp-based functional annotation (first pass)

Fetched 2023-01-10 16:34:58 GMT
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Inputs

ID Type Title Doc
lds2 File Extract ORF Proteins/lds2
taxid Integer
blastdb String[] Input blastdb databases
plasmids File Prepare_Unannotated_Sequences/plasmids
proteins File Extract ORF Proteins/proteins
raw_seqs File Prepare_Unannotated_Sequences/sequences
taxon_db File
annotation File Get ORFs/outseq
genus_list Integer[]
prot_ids_A File Extract ORF Proteins/prot_ids
prot_ids_B1 File Get off-frame ORFs/prot_ids
prot_ids_B2 File AntiFam tainted proteins I/oseqids
unicoll_cache Directory
blast_rules_db String parameter to store the literal 'blast_rules_db'
sequence_cache Directory
blast_hits_cache File (Optional)
identification_db_dir Directory Create identification BLASTdb
scatter_gather_nchunks String

Steps

ID Runs Label Doc
Map_Naming_Hits
bacterial_hit_mapping.cwl (CommandLineTool)
bacterial_hit_mapping
Remove_off_frame_ORFs
../progs/set_operation.cwl (CommandLineTool)
set_operation
Separate_Plasmid_ORFs
../progs/separate_plasmid_orfs.cwl (CommandLineTool)
separate_plasmid_orfs
Find_Naming_Protein_Hits_for_struct blastp_wnode_naming
Find_Struct_Protein_Hits_for_Plasmids blastp_wnode_struct

Outputs

ID Type Label Doc
aligns File goes to protein_alignment/Seed Search Compartments/compartments
Permalink: https://w3id.org/cwl/view/git/7cee09fb3e33c851e4e1dfc965c558b82290a785/bacterial_annot/wf_bacterial_annot_pass2.cwl