Workflow: pipeline-fastq2vcf-parallel-bwa.cwl

Fetched 2025-07-13 22:56:41 GMT

DNAseq pipeline from fastq to vcf

children parents
Workflow as SVG
  • Selected
  • Default Values
  • Nested Workflows
  • Tools
  • Inputs/Outputs

Inputs

ID Type Title Doc
dbsnp File (Optional)
sample String[]
library String (Optional)
threads Integer (Optional)
interval File (Optional)
platform String (Optional)
readgroup String[]
reference File
chunk_size Integer (Optional)
input_reads e69b8b3652ca76afcae5241ce92c2c38[]
output_file String
bwa_output_bam String
mark_secondary Boolean (Optional)
bqsr_known_sites File[]
dedup_output_bam String
qcal_output_file String
realign_output_bam String
realign_known_sites File[]
dedup_metrics_output_file String

Steps

ID Runs Label Doc
hc
../algo/hc.cwl (CommandLineTool)
bwa
../algo/bwa-mem-sort.cwl (CommandLineTool)
bqsr

Run BQSR pre+post+plot flow

dedup

run 2-pass dedup: algo LocusCollector + algo Dedup sequentially

realign
../algo/realign.cwl (CommandLineTool)
bam_merge
../algo/bam-merge.cwl (CommandLineTool)

merge sorted bam files. If input bam files are in the order of coordinate, and no overlap, mergemode 10 can be used

Outputs

ID Type Label Doc
output File
bwa_output File
qcal_output File
dedup_output File
realign_output File
qcal_plot_output File (Optional)
dedup_metrics_output File (Optional)
qcal_plot_csv_output File (Optional)
Permalink: https://w3id.org/cwl/view/git/d20382adfe7285cb517a25d95d2bcb7586546e23/pipeline/pipeline-fastq2vcf-parallel-bwa.cwl