Explore Workflows
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Trim Galore RNA-Seq pipeline paired-end
The original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for a **pair-end** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow must be used with paired-end RNA-Seq data. It performs the following steps: 1. Trim adapters from input FASTQ files 2. Use STAR to align reads from input FASTQ files according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 3. Use fastx_quality_stats to analyze input FASTQ files and generate quality statistics files 4. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 2 (after running STAR) 5. Generate BigWig file using sorted BAM file 6. Map input FASTQ files to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
Path: workflows/trim-rnaseq-pe.cwl Branch/Commit ID: b4d578c2ba4713a5a22163d9f8c7105acda1f22e |
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THOR - differential peak calling of ChIP-seq signals with replicates
What is THOR? -------------- THOR is an HMM-based approach to detect and analyze differential peaks in two sets of ChIP-seq data from distinct biological conditions with replicates. THOR performs genomic signal processing, peak calling and p-value calculation in an integrated framework. For more information please refer to: ------------------------------------- Allhoff, M., Sere K., Freitas, J., Zenke, M., Costa, I.G. (2016), Differential Peak Calling of ChIP-seq Signals with Replicates with THOR, Nucleic Acids Research, epub gkw680. |
Path: workflows/rgt-thor.cwl Branch/Commit ID: 9b4dc225c537685b9c9a32d931d3892d20953dd7 |
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wgs alignment and germline variant detection
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Path: definitions/pipelines/germline_wgs_gvcf.cwl Branch/Commit ID: 5be54bf09092c53e6c7797a875f64a360d511d7f |
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mut.cwl
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Path: tests/wf/mut.cwl Branch/Commit ID: bf93dd3e6e3261e1455530984ce045f283535d17 |
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step-valuefrom2-wf.cwl
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Path: tests/step-valuefrom2-wf.cwl Branch/Commit ID: ea9f8634e41824ac3f81c3dde698d5f0eef54f1b |
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Aligning chipseq data of mouse
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Path: definitions/subworkflows/sequence_to_bqsr_mouse.cwl Branch/Commit ID: 25aa4788dd4efb1cc8ed6f609cb7803896e4d28d |
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scatter-wf4.cwl#main
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Path: tests/wf/scatter-wf4.cwl Branch/Commit ID: 5ef2516220cd2ed327ba7966e7d812de969f4eea Packed ID: main |
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Genomic regions intersection and visualization
Genomic regions intersection and visualization ============================================== 1. Merges intervals within each of the filtered peaks files from ChIP/ATAC experiments 2. Overlaps merged intervals and assigns the nearest genes to them |
Path: workflows/intervene.cwl Branch/Commit ID: b4d578c2ba4713a5a22163d9f8c7105acda1f22e |
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kmer_top_n_extract
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Path: task_types/tt_kmer_top_n_extract.cwl Branch/Commit ID: 8cc9b995bca666c54c673a5eb8d9b8c6f8e84490 |
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strelka workflow
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Path: definitions/subworkflows/strelka_and_post_processing.cwl Branch/Commit ID: 86fbeb95ef85111f3b4c6bc2bba8f06cef64e157 |
