Explore Workflows
View already parsed workflows here or click here to add your own
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kmer_ref_compare_wnode
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![]() Path: task_types/tt_kmer_ref_compare_wnode.cwl Branch/Commit ID: 8af4e2aabf43d5e3c7162efae4ad4649df5601e2 |
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scatter-wf4.cwl#main
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![]() Path: tests/scatter-wf4.cwl Branch/Commit ID: 664835e83eb5e57eee18a04ce7b05fb9d70d77b7 Packed ID: main |
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js_output_workflow.cwl
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![]() Path: tests/wf/js_output_workflow.cwl Branch/Commit ID: 55ccde7c2fe3e7899136ce8606a341e292d7050a |
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Running cellranger count and lineage inference
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![]() Path: definitions/subworkflows/single_cell_rnaseq.cwl Branch/Commit ID: b7d9ace34664d3cedb16f2512c8a6dc6debfc8ca |
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get_spike_in_counts.cwl
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![]() Path: CWL/workflow_modules/get_spike_in_counts.cwl Branch/Commit ID: 12aba268a55088a0350b7475a255a9713c78f8ff |
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Transcripts annotation workflow
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![]() Path: workflows/TranscriptsAnnotation-i5only-wf.cwl Branch/Commit ID: 4c860f3356b3a94b07f620d5e11aa1607d3c36e1 |
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kmer_cache_retrieve
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![]() Path: task_types/tt_kmer_cache_retrieve.cwl Branch/Commit ID: cabb1a9a95244e93294727be8cf5816c38992cb0 |
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kmer_cache_store
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![]() Path: task_types/tt_kmer_cache_store.cwl Branch/Commit ID: c28cfb9882dedd3c522160f933cff1050ae24100 |
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Kallisto transcript quant pipeline paired end
This workflow runs paired end RNA-Seq reads using the kallisto quant tool against a kallisto index reference genome (see \"Kallisto index pipeline\"). The kallisto transcript-level quantified samples are then compatible with the DESeq and GSEA downstream workflows. ### __Inputs__ - Kallisto index sample (of experimental organism) - R1/R2 FASTQ files of RNA-Seq read data - number of threads to use for multithreading processes ### __Outputs__ - kallisto quant file (transcript estimate tsv) ### __Data Analysis Steps__ 1. cwl calls dockercontainer robertplayer/scidap-kallisto to pseudo align reads using `kallisto quant`. 2. abundance tsv is formatted, and additional files are produced for gene and common TSS counts for use in differential expression analysis 3. read and alignment metrics are calculated for the sample piechart, and output to the overview.md file ### __References__ - Bray, N. L., Pimentel, H., Melsted, P. & Pachter, L. Near-optimal probabilistic RNA-seq quantification, Nature Biotechnology 34, 525-527(2016), doi:10.1038/nbt.3519 |
![]() Path: workflows/kallisto-quant-pe.cwl Branch/Commit ID: 261c0232a7a40880f2480b811ed2d7e89c463869 |
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env-wf1.cwl
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![]() Path: v1.0/v1.0/env-wf1.cwl Branch/Commit ID: 4fd45edb9531a03223c18a586e32d0baf0d5acb2 |