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umi duplex alignment fastq workflow
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![]() Path: definitions/pipelines/umi_duplex_alignment.cwl Branch/Commit ID: d2c2f2eb846ae2e9cdcab46e3bb88e42126cb3f5 |
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Bismark Methylation SE
Sequence reads are first cleaned from adapters and transformed into fully bisulfite-converted forward (C->T) and reverse read (G->A conversion of the forward strand) versions, before they are aligned to similarly converted versions of the genome (also C->T and G->A converted). Sequence reads that produce a unique best alignment from the four alignment processes against the bisulfite genomes (which are running in parallel) are then compared to the normal genomic sequence and the methylation state of all cytosine positions in the read is inferred. A read is considered to align uniquely if an alignment has a unique best alignment score (as reported by the AS:i field). If a read produces several alignments with the same number of mismatches or with the same alignment score (AS:i field), a read (or a read-pair) is discarded altogether. On the next step we extract the methylation call for every single C analysed. The position of every single C will be written out to a new output file, depending on its context (CpG, CHG or CHH), whereby methylated Cs will be labelled as forward reads (+), non-methylated Cs as reverse reads (-). The output of the methylation extractor is then transformed into a bedGraph and coverage file. The bedGraph counts output is then used to generate a genome-wide cytosine report which reports the number on every single CpG (optionally every single cytosine) in the genome, irrespective of whether it was covered by any reads or not. As this type of report is informative for cytosines on both strands the output may be fairly large (~46mn CpG positions or >1.2bn total cytosine positions in the human genome). |
![]() Path: workflows/bismark-methylation-se.cwl Branch/Commit ID: 675a3ff982091faf304931e9261aacdbabf51702 |
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echo-wf-default.cwl
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![]() Path: cwltool/schemas/v1.0/v1.0/echo-wf-default.cwl Branch/Commit ID: b82ce7ae901a54c7a062fd5eefd8d5ceb5a4d684 |
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816_wf.cwl
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![]() Path: tests/wf/816_wf.cwl Branch/Commit ID: 55ccde7c2fe3e7899136ce8606a341e292d7050a |
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Motif Finding with HOMER with target and background regions from peaks
Motif Finding with HOMER with target and background regions from peaks --------------------------------------------------- HOMER contains a novel motif discovery algorithm that was designed for regulatory element analysis in genomics applications (DNA only, no protein). It is a differential motif discovery algorithm, which means that it takes two sets of sequences and tries to identify the regulatory elements that are specifically enriched in on set relative to the other. It uses ZOOPS scoring (zero or one occurrence per sequence) coupled with the hypergeometric enrichment calculations (or binomial) to determine motif enrichment. HOMER also tries its best to account for sequenced bias in the dataset. It was designed with ChIP-Seq and promoter analysis in mind, but can be applied to pretty much any nucleic acids motif finding problem. For more information please refer to: ------------------------------------- [Official documentation](http://homer.ucsd.edu/homer/motif/) |
![]() Path: workflows/homer-motif-analysis-peak.cwl Branch/Commit ID: ebbf23764ede324cabc064bd50647c1f643726fa |
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PGAP Pipeline, simple user input, PGAPX-134
PGAP pipeline for external usage, powered via containers, simple user input: (FASTA + yaml only, no template) |
![]() Path: pgap.cwl Branch/Commit ID: 5461e63dc4714bb81e1c9f58e436c8465107a199 |
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kmer_build_tree
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![]() Path: task_types/tt_kmer_build_tree.cwl Branch/Commit ID: a7fced3ed8c839272c8f3a8db9da7bc8cd50271f |
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Single-Cell RNA-Seq Trajectory Analysis
Single-Cell RNA-Seq Trajectory Analysis Infers developmental trajectories and pseudotime from cells clustered by similarity of gene expression data. |
![]() Path: workflows/sc-rna-trajectory.cwl Branch/Commit ID: 675a3ff982091faf304931e9261aacdbabf51702 |
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io-any-wf-1.cwl
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![]() Path: tests/io-any-wf-1.cwl Branch/Commit ID: 31ec48a8d81ef7c1b2c5e9c0a19e7623efe4a1e2 |
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kmer_compare_wnode
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![]() Path: task_types/tt_kmer_compare_wnode.cwl Branch/Commit ID: e6fd7898b71a89b667d2eb38f412999920be5902 |