Explore Workflows
View already parsed workflows here or click here to add your own
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revsort.cwl
Reverse the lines in a document, then sort those lines. |
![]() Path: tests/wf/revsort.cwl Branch/Commit ID: d6000d32f6c8fbd26421a2d30d79b28901d58fb0 |
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io-union-input-default-wf.cwl
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![]() Path: tests/io-union-input-default-wf.cwl Branch/Commit ID: ea9f8634e41824ac3f81c3dde698d5f0eef54f1b |
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mut3.cwl
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![]() Path: tests/wf/mut3.cwl Branch/Commit ID: 8ef515037de411abd2f84b569ad4d4a4f7a2c7a0 |
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PCA - Principal Component Analysis
Principal Component Analysis --------------- Principal component analysis (PCA) is a statistical procedure that uses an orthogonal transformation to convert a set of observations of possibly correlated variables (entities each of which takes on various numerical values) into a set of values of linearly uncorrelated variables called principal components. The calculation is done by a singular value decomposition of the (centered and possibly scaled) data matrix, not by using eigen on the covariance matrix. This is generally the preferred method for numerical accuracy. |
![]() Path: workflows/pca.cwl Branch/Commit ID: a68821bf3a9ceadc3b2ffbb535d601d9a645b377 |
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Generate genome indices for STAR & bowtie
Creates indices for: * [STAR](https://github.com/alexdobin/STAR) v2.5.3a (03/17/2017) PMID: [23104886](https://www.ncbi.nlm.nih.gov/pubmed/23104886) * [bowtie](http://bowtie-bio.sourceforge.net/tutorial.shtml) v1.2.0 (12/30/2016) It performs the following steps: 1. `STAR --runMode genomeGenerate` to generate indices, based on [FASTA](http://zhanglab.ccmb.med.umich.edu/FASTA/) and [GTF](http://mblab.wustl.edu/GTF2.html) input files, returns results as an array of files 2. Outputs indices as [Direcotry](http://www.commonwl.org/v1.0/CommandLineTool.html#Directory) data type 3. Separates *chrNameLength.txt* file from Directory output 4. `bowtie-build` to generate indices requires genome [FASTA](http://zhanglab.ccmb.med.umich.edu/FASTA/) file as input, returns results as a group of main and secondary files |
![]() Path: workflows/genome-indices.cwl Branch/Commit ID: c5bae2ca862c764911b83d1f15ff6af4e2a0db28 |
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basename-fields-test.cwl
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![]() Path: cwltool/schemas/v1.0/v1.0/basename-fields-test.cwl Branch/Commit ID: 7c7615c44b80f8e76e659433f8c7875603ae0b25 |
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xenbase-chipseq-se.cwl
XenBase workflow for analysing ChIP-Seq single-end data |
![]() Path: workflows/xenbase-chipseq-se.cwl Branch/Commit ID: 4b8bb1a1ec39056253ca8eee976078e51f4a954e |
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rnaseq-se.cwl
RNA-Seq basic analysis workflow for single-read experiment. |
![]() Path: workflows/rnaseq-se.cwl Branch/Commit ID: 4b8bb1a1ec39056253ca8eee976078e51f4a954e |
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RNA-Seq pipeline single-read stranded mitochondrial
Slightly changed original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for **strand specific single-read** experiment. An additional steps were added to map data to mitochondrial chromosome only and then merge the output. Experiment files in [FASTQ](http://maq.sourceforge.net/fastq.shtml) format either compressed or not can be used. Current workflow should be used only with single-read strand specific RNA-Seq data. It performs the following steps: 1. `STAR` to align reads from input FASTQ file according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 2. `fastx_quality_stats` to analyze input FASTQ file and generate quality statistics file 3. `samtools sort` to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ file to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using `GEEP` reads-counting utility; export results to file |
![]() Path: workflows/rnaseq-se-dutp-mitochondrial.cwl Branch/Commit ID: a68821bf3a9ceadc3b2ffbb535d601d9a645b377 |
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Immunotherapy Workflow
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![]() Path: definitions/pipelines/immuno.cwl Branch/Commit ID: 8dc462a7d9ba1479f764682af99c69d8574cb3dc |