Explore Workflows
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EMG pipeline's QIIME workflow
Step 1: Set environment PYTHONPATH, QIIME_ROOT, PATH Step 2: Run QIIME script pick_closed_reference_otus.py ${python} ${qiimeDir}/bin/pick_closed_reference_otus.py -i $1 -o $2 -r ${qiimeDir}/gg_13_8_otus/rep_set/97_otus.fasta -t ${qiimeDir}/gg_13_8_otus/taxonomy/97_otu_taxonomy.txt -p ${qiimeDir}/cr_otus_parameters.txt Step 3: Convert new biom format to old biom format (json) ${qiimeDir}/bin/biom convert -i ${resultDir}/cr_otus/otu_table.biom -o ${resultDir}/cr_otus/${infileBase}_otu_table_json.biom --table-type=\"OTU table\" --to-json Step 4: Convert new biom format to a classic OTU table. ${qiimeDir}/bin/biom convert -i ${resultDir}/cr_otus/otu_table.biom -o ${resultDir}/cr_otus/${infileBase}_otu_table.txt --to-tsv --header-key taxonomy --table-type \"OTU table\" Step 5: Create otu summary ${qiimeDir}/bin/biom summarize-table -i ${resultDir}/cr_otus/otu_table.biom -o ${resultDir}/cr_otus/${infileBase}_otu_table_summary.txt Step 6: Move one of the result files mv ${resultDir}/cr_otus/otu_table.biom ${resultDir}/cr_otus/${infileBase}_otu_table_hdf5.biom Step 7: Create a list of observations awk '{print $1}' ${resultDir}/cr_otus/${infileBase}_otu_table.txt | sed '/#/d' > ${resultDir}/cr_otus/${infileBase}_otu_observations.txt Step 8: Create a phylogenetic tree by pruning GreenGenes and keeping observed otus ${python} ${qiimeDir}/bin/filter_tree.py -i ${qiimeDir}/gg_13_8_otus/trees/97_otus.tree -t ${resultDir}/cr_otus/${infileBase}_otu_observations.txt -o ${resultDir}/cr_otus/${infileBase}_pruned.tree |
![]() Path: workflows/qiime-workflow.cwl Branch/Commit ID: 7be1be85bd4f8dc72a9f1572a6637bb1f2536bed |
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Build Bowtie indices
Workflow runs [Bowtie](http://bowtie-bio.sourceforge.net/tutorial.shtml) v1.2.0 (12/30/2016) to build indices for reference genome provided in a single FASTA file as fasta_file input. Generated indices are saved in a folder with the name that corresponds to the input genome |
![]() Path: workflows/bowtie-index.cwl Branch/Commit ID: 7ced5a5259dbd8b3fc64456beaeffd44f4a24081 |
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filter-pcr-artifacts.cwl
DNase-seq - map - Filter PCR Artifacts |
![]() Path: v1.0/map/filter-pcr-artifacts.cwl Branch/Commit ID: c50b80e9b0eaaf4613f9feca44b3463bbfd288d5 |
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any-type-compat.cwl
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![]() Path: cwltool/schemas/v1.0/v1.0/any-type-compat.cwl Branch/Commit ID: 665141f319e6b23bd9924b14844f2e979f141944 |
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conflict-wf.cwl#collision
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![]() Path: cwltool/schemas/v1.0/v1.0/conflict-wf.cwl Branch/Commit ID: e9c83739a93fa0b18f8dea2f98b632a9e32725c9 Packed ID: collision |
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cond-wf-005.1.cwl
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![]() Path: testdata/cond-wf-005.1.cwl Branch/Commit ID: c1875d54dedc41b1d2fa08634dcf1caa8f1bc631 |
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cond-wf-004.cwl
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![]() Path: tests/conditionals/cond-wf-004.cwl Branch/Commit ID: e62f99dd79d6cb9c157cceb458f74200da84f6e9 |
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workflow_input_format_expr_v1_2.cwl
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![]() Path: testdata/workflow_input_format_expr_v1_2.cwl Branch/Commit ID: c1875d54dedc41b1d2fa08634dcf1caa8f1bc631 |
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personal_genome_input_wf.cwl
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![]() Path: workflow/personal_genome_input_wf.cwl Branch/Commit ID: 3fd55a819cda2a1fc48957b5daa2bebdaee843fb |
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Bisulfite alignment and QC
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![]() Path: definitions/pipelines/bisulfite.cwl Branch/Commit ID: 49508a2757ff2f49f1c200774a38af1c12b531bf |