Explore Workflows
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Graph | Name | Retrieved From | View |
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Quality assessment, amplicon classification and functional prediction
Workflow for quality assessment of paired reads and classification using NGTax 2.0 and functional annotation using picrust2. In addition files are exported to their respective subfolders for easier data management in a later stage. Steps: - FastQC (read quality control) - NGTax 2.0 - Picrust 2 - Export module for ngtax |
https://git.wageningenur.nl/unlock/cwl.git
Path: cwl/workflows/workflow_ngtax_picrust2.cwl Branch/Commit ID: b9097b82e6ab6f2c9496013ce4dd6877092956a0 |
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kmer_top_n_extract
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https://github.com/ncbi/pgap.git
Path: task_types/tt_kmer_top_n_extract.cwl Branch/Commit ID: 803f6367d1b279a7b6dc1a4e8ae43f1bbec9f760 |
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tt_univec_wnode.cwl
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https://github.com/ncbi/pgap.git
Path: task_types/tt_univec_wnode.cwl Branch/Commit ID: 7f857f7f2d7c080d27c775b67a6d6f7d94bce31f |
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scatter-wf1.cwl
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https://github.com/common-workflow-language/cwltool.git
Path: cwltool/schemas/v1.0/v1.0/scatter-wf1.cwl Branch/Commit ID: 047e69bb169e79fad6a7285ee798c4ecec3b218b |
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allele-rnaseq-pe.cwl
Allele specific RNA-Seq paired-end workflow |
https://github.com/datirium/workflows.git
Path: workflows/allele-rnaseq-pe.cwl Branch/Commit ID: cf107bc24a37883ef01b959fd89c19456aaecc02 |
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dedup-3-pass-distr.cwl
run 3-pass dedup: algo LocusCollector + algo Dedup output_dup_read_name + algo Dedup dedup_by_read_name sequentially in distributed mode |
https://github.com/sentieon/sentieon-cwl.git
Path: stage/dedup-3-pass-distr.cwl Branch/Commit ID: d20382adfe7285cb517a25d95d2bcb7586546e23 |
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DESeq - differential gene expression analysis
Differential gene expression analysis ===================================== Differential gene expression analysis based on the negative binomial distribution Estimate variance-mean dependence in count data from high-throughput sequencing assays and test for differential expression based on a model using the negative binomial distribution. DESeq1 ------ High-throughput sequencing assays such as RNA-Seq, ChIP-Seq or barcode counting provide quantitative readouts in the form of count data. To infer differential signal in such data correctly and with good statistical power, estimation of data variability throughout the dynamic range and a suitable error model are required. Simon Anders and Wolfgang Huber propose a method based on the negative binomial distribution, with variance and mean linked by local regression and present an implementation, [DESeq](http://bioconductor.org/packages/release/bioc/html/DESeq.html), as an R/Bioconductor package DESeq2 ------ In comparative high-throughput sequencing assays, a fundamental task is the analysis of count data, such as read counts per gene in RNA-seq, for evidence of systematic changes across experimental conditions. Small replicate numbers, discreteness, large dynamic range and the presence of outliers require a suitable statistical approach. [DESeq2](http://www.bioconductor.org/packages/release/bioc/html/DESeq2.html), a method for differential analysis of count data, using shrinkage estimation for dispersions and fold changes to improve stability and interpretability of estimates. This enables a more quantitative analysis focused on the strength rather than the mere presence of differential expression. |
https://github.com/datirium/workflows.git
Path: workflows/deseq.cwl Branch/Commit ID: 4ab9399a4777610a579ea2c259b9356f27641dcc |
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extract_gencoll_ids
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https://github.com/ncbi/pgap.git
Path: task_types/tt_extract_gencoll_ids.cwl Branch/Commit ID: 7f857f7f2d7c080d27c775b67a6d6f7d94bce31f |
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align_sort_sa
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https://github.com/ncbi/pgap.git
Path: task_types/tt_align_sort_sa.cwl Branch/Commit ID: 61eaea2f746c8a1fc2a2b731056b068e28ca4e20 |
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PCA - Principal Component Analysis
Principal Component Analysis --------------- Principal component analysis (PCA) is a statistical procedure that uses an orthogonal transformation to convert a set of observations of possibly correlated variables (entities each of which takes on various numerical values) into a set of values of linearly uncorrelated variables called principal components. The calculation is done by a singular value decomposition of the (centered and possibly scaled) data matrix, not by using eigen on the covariance matrix. This is generally the preferred method for numerical accuracy. |
https://github.com/datirium/workflows.git
Path: workflows/pca.cwl Branch/Commit ID: 4ab9399a4777610a579ea2c259b9356f27641dcc |