Explore Workflows
View already parsed workflows here or click here to add your own
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downsample unaligned BAM and align
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![]() Path: definitions/subworkflows/downsampled_alignment.cwl Branch/Commit ID: 0a9a4ce83b49ed4e7eee5bcc09d83725136a36b0 |
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mut2.cwl
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![]() Path: tests/wf/mut2.cwl Branch/Commit ID: 55ccde7c2fe3e7899136ce8606a341e292d7050a |
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step-valuefrom-wf.cwl
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![]() Path: cwltool/schemas/v1.0/v1.0/step-valuefrom-wf.cwl Branch/Commit ID: 2256a30d0c1365b30e0a7338fb883c74674fcd25 |
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schemadef-wf.cwl
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![]() Path: cwltool/schemas/v1.0/v1.0/schemadef-wf.cwl Branch/Commit ID: a858bb4db58ef2df17b4856294ad7904643c5c6e |
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RNA-Seq pipeline single-read
The original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for a **single-read** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the single-read RNA-Seq data. It performs the following steps: 1. Use STAR to align reads from input FASTQ file according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 2. Use fastx_quality_stats to analyze input FASTQ file and generate quality statistics file 3. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ file to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
![]() Path: workflows/rnaseq-se.cwl Branch/Commit ID: 7eef0294395d83ff0765fce61726a59d71126422 |
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EMG pipeline's QIIME workflow
Step 1: Set environment PYTHONPATH, QIIME_ROOT, PATH Step 2: Run QIIME script pick_closed_reference_otus.py ${python} ${qiimeDir}/bin/pick_closed_reference_otus.py -i $1 -o $2 -r ${qiimeDir}/gg_13_8_otus/rep_set/97_otus.fasta -t ${qiimeDir}/gg_13_8_otus/taxonomy/97_otu_taxonomy.txt -p ${qiimeDir}/cr_otus_parameters.txt Step 3: Convert new biom format to old biom format (json) ${qiimeDir}/bin/biom convert -i ${resultDir}/cr_otus/otu_table.biom -o ${resultDir}/cr_otus/${infileBase}_otu_table_json.biom --table-type=\"OTU table\" --to-json Step 4: Convert new biom format to a classic OTU table. ${qiimeDir}/bin/biom convert -i ${resultDir}/cr_otus/otu_table.biom -o ${resultDir}/cr_otus/${infileBase}_otu_table.txt --to-tsv --header-key taxonomy --table-type \"OTU table\" Step 5: Create otu summary ${qiimeDir}/bin/biom summarize-table -i ${resultDir}/cr_otus/otu_table.biom -o ${resultDir}/cr_otus/${infileBase}_otu_table_summary.txt Step 6: Move one of the result files mv ${resultDir}/cr_otus/otu_table.biom ${resultDir}/cr_otus/${infileBase}_otu_table_hdf5.biom Step 7: Create a list of observations awk '{print $1}' ${resultDir}/cr_otus/${infileBase}_otu_table.txt | sed '/#/d' > ${resultDir}/cr_otus/${infileBase}_otu_observations.txt Step 8: Create a phylogenetic tree by pruning GreenGenes and keeping observed otus ${python} ${qiimeDir}/bin/filter_tree.py -i ${qiimeDir}/gg_13_8_otus/trees/97_otus.tree -t ${resultDir}/cr_otus/${infileBase}_otu_observations.txt -o ${resultDir}/cr_otus/${infileBase}_pruned.tree |
![]() Path: workflows/qiime-workflow.cwl Branch/Commit ID: 7be1be85bd4f8dc72a9f1572a6637bb1f2536bed |
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Build Bowtie indices
Workflow runs [Bowtie](http://bowtie-bio.sourceforge.net/tutorial.shtml) v1.2.0 (12/30/2016) to build indices for reference genome provided in a single FASTA file as fasta_file input. Generated indices are saved in a folder with the name that corresponds to the input genome |
![]() Path: workflows/bowtie-index.cwl Branch/Commit ID: 7ced5a5259dbd8b3fc64456beaeffd44f4a24081 |
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filter-pcr-artifacts.cwl
DNase-seq - map - Filter PCR Artifacts |
![]() Path: v1.0/map/filter-pcr-artifacts.cwl Branch/Commit ID: c50b80e9b0eaaf4613f9feca44b3463bbfd288d5 |
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any-type-compat.cwl
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![]() Path: cwltool/schemas/v1.0/v1.0/any-type-compat.cwl Branch/Commit ID: 665141f319e6b23bd9924b14844f2e979f141944 |
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conflict-wf.cwl#collision
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![]() Path: cwltool/schemas/v1.0/v1.0/conflict-wf.cwl Branch/Commit ID: e9c83739a93fa0b18f8dea2f98b632a9e32725c9 Packed ID: collision |