Explore Workflows
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Graph | Name | Retrieved From | View |
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metabarcode (gene amplicon) analysis for fastq files
protein - qc, preprocess, annotation, index, abundance |
![]() Path: CWL/Workflows/metabarcode-fasta.workflow.cwl Branch/Commit ID: d9cf22cd615542c94f7974e8bce4cf29b24d985f |
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conflict-wf.cwl#collision
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![]() Path: tests/conflict-wf.cwl Branch/Commit ID: 1f3ef888d9ef2306c828065c460c1800604f0de4 Packed ID: collision |
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Detect DoCM variants
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![]() Path: definitions/subworkflows/docm_germline.cwl Branch/Commit ID: e59c77629936fad069007ba642cad49fef7ad29f |
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RNA-Seq pipeline single-read strand specific
Note: should be updated The original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for **strand specific single-read** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the single-read RNA-Seq data. It performs the following steps: 1. Use STAR to align reads from input FASTQ file according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 2. Use fastx_quality_stats to analyze input FASTQ file and generate quality statistics file 3. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ file to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
![]() Path: workflows/rnaseq-se-dutp.cwl Branch/Commit ID: a68821bf3a9ceadc3b2ffbb535d601d9a645b377 |
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ani.cwl
Perform taxonomic identification tasks on an input genome |
![]() Path: ani.cwl Branch/Commit ID: bba6c580ab88e077f6aa2c2ee7c73159f3f9156e |
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RNA-Seq pipeline single-read
The original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for a **single-read** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the single-read RNA-Seq data. It performs the following steps: 1. Use STAR to align reads from input FASTQ file according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 2. Use fastx_quality_stats to analyze input FASTQ file and generate quality statistics file 3. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ file to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
![]() Path: workflows/rnaseq-se.cwl Branch/Commit ID: c5bae2ca862c764911b83d1f15ff6af4e2a0db28 |
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kmer_top_n_extract
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![]() Path: task_types/tt_kmer_top_n_extract.cwl Branch/Commit ID: 9e43bc5cff985574e1f8135d4c50b5a347517c9e |
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umi duplex alignment workflow
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![]() Path: definitions/subworkflows/duplex_alignment.cwl Branch/Commit ID: c711498c04d6b8ddf92ddceb6219f074765f7993 |
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parallel_no_threshold.cwl
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![]() Path: workflows/parallel_no_threshold.cwl Branch/Commit ID: 0f9e76d981ca7b100fb9e170519b427926e590a0 |
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rnaseq-se.cwl
Runs RNA-Seq BioWardrobe basic analysis with single-end data file. |
![]() Path: workflows/rnaseq-se.cwl Branch/Commit ID: ea2a2ab57710fcf067f67305f3dd6ad29094da1a |