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Trim Galore RNA-Seq pipeline paired-end
The original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for a **pair-end** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the single-end RNA-Seq data. It performs the following steps: 1. Trim adapters from input FASTQ files 2. Use STAR to align reads from input FASTQ files according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 3. Use fastx_quality_stats to analyze input FASTQ files and generate quality statistics files 4. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ files to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
![]() Path: workflows/trim-rnaseq-pe.cwl Branch/Commit ID: e99e80a2c19682d59947bde04a892d7b6d90091c |
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Create Genomic Collection for Bacterial Pipeline, ASN.1 input
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![]() Path: genomic_source/wf_genomic_source_asn.cwl Branch/Commit ID: 2afb5ebafd1353ba063cc74ee9a7eaf347afce5c |
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Assess the statistical description of calibration events
When DPPS receives a new DL0 data product, the CalibPipe is triggered to process the calibration events. The CalibPipe performs charge integration and peak time extraction for the entire set of calibration events, and computes aggregated time-series statistics, including the mean, median, and standard deviation. Using these aggregated statistics, the CalibPipe identifies faulty camera pixels, such as those affected by starlight, by applying various outlier detection criteria. Time periods with a significant number of faulty pixels, exceeding a predefined threshold, are flagged as invalid. A refined treatment can then be applied to these time periods to account for the issues. |
![]() Path: workflows/telescope/camera/uc-120-2.21-assess-statistical-description.cwl Branch/Commit ID: 81949412f693d624e10883485ac2758c9067a50f |
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HS Metrics workflow
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![]() Path: definitions/subworkflows/hs_metrics.cwl Branch/Commit ID: ec45fad68ca10fb64d5c58e704991b146dc31d28 |
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gathered exome alignment and somatic variant detection for cle purpose
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![]() Path: definitions/pipelines/somatic_exome_cle_gathered.cwl Branch/Commit ID: d3e4bf55753cd92f97537c7d701187ea92d1e5f0 |
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default-dir5.cwl
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![]() Path: tests/wf/default-dir5.cwl Branch/Commit ID: 8ef515037de411abd2f84b569ad4d4a4f7a2c7a0 |
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Filter Protein Seeds; Find ProSplign Alignments
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![]() Path: protein_alignment/wf_compart_filter_prosplign.cwl Branch/Commit ID: 8ea3637b0f11eac1ea5599c41d74e00d85fb778d |
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Gathered Downsample and HaplotypeCaller
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![]() Path: definitions/pipelines/gathered_downsample_and_recall.cwl Branch/Commit ID: 87faba2fff8007ecc95160729b1c7cd0376e46f2 |
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Tag enrichment heatmap and density profile around regions of interest
Generates tag density heatmap and histogram for the centered list of features in a headerless regions file. - If provided regions file is a gene list with the following columns `chrom start end name score strand` set `Gene TSS` as a re-centering criteria. - If provided regions file is a peak list with the following columns `chrom start end name` set `Peak Center` as a re-centering criteria. `score` column is always ignored. |
![]() Path: workflows/heatmap.cwl Branch/Commit ID: 46d3d403ddb240d5a8f4f31ab992b6d6a2686745 |
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umi per-lane alignment subworkflow
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![]() Path: definitions/subworkflows/umi_alignment.cwl Branch/Commit ID: ffab5424bb8b5905aecf6f8e2e6387da7f3df562 |