Explore Workflows
View already parsed workflows here or click here to add your own
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bam_readcount workflow
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![]() Path: definitions/subworkflows/bam_readcount.cwl Branch/Commit ID: ec45fad68ca10fb64d5c58e704991b146dc31d28 |
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Raw sequence data to BQSR
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![]() Path: definitions/subworkflows/sequence_to_bqsr.cwl Branch/Commit ID: 25aa4788dd4efb1cc8ed6f609cb7803896e4d28d |
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mut.cwl
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![]() Path: tests/wf/mut.cwl Branch/Commit ID: 89ccbfc53ff3bb6abe2eb90bb7e0091c54c18f5c |
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RNA-Seq pipeline paired-end stranded mitochondrial
Slightly changed original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for **strand specific pair-end** experiment. An additional steps were added to map data to mitochondrial chromosome only and then merge the output. Experiment files in [FASTQ](http://maq.sourceforge.net/fastq.shtml) format either compressed or not can be used. Current workflow should be used only with the pair-end strand specific RNA-Seq data. It performs the following steps: 1. `STAR` to align reads from input FASTQ file according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 2. `fastx_quality_stats` to analyze input FASTQ file and generate quality statistics file 3. `samtools sort` to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ file to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using `GEEP` reads-counting utility; export results to file |
![]() Path: workflows/rnaseq-pe-dutp-mitochondrial.cwl Branch/Commit ID: 7eef0294395d83ff0765fce61726a59d71126422 |
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tt_blastn_wnode
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![]() Path: task_types/tt_blastn_wnode.cwl Branch/Commit ID: c6e7e18969c761803c38762ad6ee91b0001c52e2 |
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tt_univec_wnode.cwl
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![]() Path: task_types/tt_univec_wnode.cwl Branch/Commit ID: 61e3752f1f5e2ee498fa024c235226f8580be942 |
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count-lines1-wf.cwl
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![]() Path: tests/count-lines1-wf.cwl Branch/Commit ID: c7c97715b400ff2194aa29fc211d3401cea3a9bf |
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tt_kmer_compare_wnode
Pairwise comparison |
![]() Path: task_types/tt_kmer_compare_wnode.cwl Branch/Commit ID: 33dcc054a8718edad26440f085d73b7c5d7b7871 |
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Feature expression merge - combines feature expression from several experiments
Feature expression merge - combines feature expression from several experiments ========================================================================= Workflows merges RPKM (by default) gene expression from several experiments based on the values from GeneId, Chrom, TxStart, TxEnd and Strand columns (by default). Reported unique columns are renamed based on the experiments names. |
![]() Path: workflows/feature-merge.cwl Branch/Commit ID: b4d578c2ba4713a5a22163d9f8c7105acda1f22e |
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WES GATK4
Whole Exome Sequence analysis GATK4 Preprocessing |
![]() Path: workflows/exomeseq-gatk4.cwl Branch/Commit ID: 66b46c15d266fdf6a1faabd8d2f1b257f3438efc |