Explore Workflows
View already parsed workflows here or click here to add your own
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Build Bowtie indices
Workflow runs [Bowtie](http://bowtie-bio.sourceforge.net/tutorial.shtml) v1.2.0 (12/30/2016) to build indices for reference genome provided in a single FASTA file as fasta_file input. Generated indices are saved in a folder with the name that corresponds to the input genome |
Path: workflows/bowtie-index.cwl Branch/Commit ID: 2005c6b7f1bff6247d015ff6c116bd9ec97158bb |
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protein annotation
Proteins - predict, filter, cluster, identify, annotate |
Path: CWL/Workflows/protein-filter-annotation.workflow.cwl Branch/Commit ID: 963ca6a73a9f8879d57c08fa59548f98f28e755a |
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Cell Ranger ARC Count Gene Expression + ATAC
Cell Ranger ARC Count Gene Expression + ATAC ============================================ |
Path: workflows/cellranger-arc-count.cwl Branch/Commit ID: ed15394da780edc9bfea2cde9e9cee8b7f267bf4 |
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Motif Finding with HOMER with target and background regions from peaks
Motif Finding with HOMER with target and background regions from peaks --------------------------------------------------- HOMER contains a novel motif discovery algorithm that was designed for regulatory element analysis in genomics applications (DNA only, no protein). It is a differential motif discovery algorithm, which means that it takes two sets of sequences and tries to identify the regulatory elements that are specifically enriched in on set relative to the other. It uses ZOOPS scoring (zero or one occurrence per sequence) coupled with the hypergeometric enrichment calculations (or binomial) to determine motif enrichment. HOMER also tries its best to account for sequenced bias in the dataset. It was designed with ChIP-Seq and promoter analysis in mind, but can be applied to pretty much any nucleic acids motif finding problem. For more information please refer to: ------------------------------------- [Official documentation](http://homer.ucsd.edu/homer/motif/) |
Path: workflows/homer-motif-analysis-peak.cwl Branch/Commit ID: 2005c6b7f1bff6247d015ff6c116bd9ec97158bb |
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RNA-Seq pipeline single-read stranded mitochondrial
Slightly changed original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for **strand specific single-read** experiment. An additional steps were added to map data to mitochondrial chromosome only and then merge the output. Experiment files in [FASTQ](http://maq.sourceforge.net/fastq.shtml) format either compressed or not can be used. Current workflow should be used only with single-read strand specific RNA-Seq data. It performs the following steps: 1. `STAR` to align reads from input FASTQ file according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 2. `fastx_quality_stats` to analyze input FASTQ file and generate quality statistics file 3. `samtools sort` to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ file to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using `GEEP` reads-counting utility; export results to file |
Path: workflows/rnaseq-se-dutp-mitochondrial.cwl Branch/Commit ID: 4ab9399a4777610a579ea2c259b9356f27641dcc |
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Single-cell Assign Cell Types
Single-cell Assign Cell Types ============================= Assigns cell types to Seurat clusters. |
Path: workflows/sc-assign-cell-types.cwl Branch/Commit ID: 10ce6e113f749c7bd725e426445220c3bdc5ddf1 |
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03-map-pe.cwl
ChIP-seq 03 mapping - reads: PE |
Path: v1.0/ChIP-seq_pipeline/03-map-pe.cwl Branch/Commit ID: ffdc6d7b155fe301cd49b6e499097cce966159ef |
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scatterfail.cwl
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Path: tests/wf/scatterfail.cwl Branch/Commit ID: 12993a6eb60f5ccb4edbe77cb6de661cfc496090 |
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align_sort_sa
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Path: task_types/tt_align_sort_sa.cwl Branch/Commit ID: 4b73bfeb967ee9f57a0410276f7c39e784f0846f |
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tt_kmer_top_n.cwl
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Path: task_types/tt_kmer_top_n.cwl Branch/Commit ID: 2353ee2550529ca5b0705c94b32022a21713db18 |
