Explore Workflows
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Graph | Name | Retrieved From | View |
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RNA-Seq pipeline paired-end
The original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for a **paired-end** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the paired-end RNA-Seq data. It performs the following steps: 1. Use STAR to align reads from input FASTQ files according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 2. Use fastx_quality_stats to analyze input FASTQ files and generate quality statistics files 3. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 4. Generate BigWig file on the base of sorted BAM file 5. Map input FASTQ files to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 6. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
![]() Path: workflows/rnaseq-pe.cwl Branch/Commit ID: 60854b5d299df91e135e05d02f4be61f6a310fbc |
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workflow_mock_ngtax.cwl
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![]() Path: cwl/workflows/workflow_mock_ngtax.cwl Branch/Commit ID: 60fafdfbec9b39c860945ef4634e0c28cb5e976c |
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iwdr_with_nested_dirs.cwl
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![]() Path: cwltool/schemas/v1.0/v1.0/iwdr_with_nested_dirs.cwl Branch/Commit ID: f997d13af87216e9b5048c732a511053c7ba714c |
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RNA-Seq pipeline paired-end strand specific
The original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for a **paired-end** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the paired-end RNA-Seq data. It performs the following steps: 1. Use STAR to align reads from input FASTQ files according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 2. Use fastx_quality_stats to analyze input FASTQ files and generate quality statistics files 3. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 4. Generate BigWig file on the base of sorted BAM file 5. Map input FASTQ files to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 6. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
![]() Path: workflows/rnaseq-pe-dutp.cwl Branch/Commit ID: bfa3843bcf36125ff258d6314f64b41336f06e6b |
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count-lines6-wf.cwl
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![]() Path: cwltool/schemas/v1.0/v1.0/count-lines6-wf.cwl Branch/Commit ID: f997d13af87216e9b5048c732a511053c7ba714c |
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Apply filters to VCF file
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![]() Path: definitions/subworkflows/germline_filter_vcf.cwl Branch/Commit ID: d3e4bf55753cd92f97537c7d701187ea92d1e5f0 |
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Prepare user input
Prepare user input for NCBI-PGAP pipeline |
![]() Path: prepare_user_input2.cwl Branch/Commit ID: 5ec226c941562124032ca6861bc8d1aeabf9d91a |
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Run pindel on provided region
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![]() Path: definitions/subworkflows/pindel_region.cwl Branch/Commit ID: e8b7759826df40b8bb821b40b15aea960a4951c4 |
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hmmsearch_wnode and gpx_qdump combined workflow to apply scatter/gather
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![]() Path: task_types/tt_hmmsearch_wnode_plus_qdump.cwl Branch/Commit ID: 1a6b9e5dea09caa0debbaff30ca39005dfa5e4d4 |
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process VCF workflow
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![]() Path: definitions/subworkflows/strelka_process_vcf.cwl Branch/Commit ID: c625e05eefb1754353c1bdfa46c01dc61e6233dd |