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EMG pipeline v3.0 (single end version)
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![]() Path: workflows/emg-pipeline-v3.cwl Branch/Commit ID: caea457b17388fdc5cb088364c194504ae736bdd |
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heatmap-prepare.cwl
Workflow runs homer-make-tag-directory.cwl tool using scatter for the following inputs - bam_file - fragment_size - total_reads `dotproduct` is used as a `scatterMethod`, so one element will be taken from each array to construct each job: 1) bam_file[0] fragment_size[0] total_reads[0] 2) bam_file[1] fragment_size[1] total_reads[1] ... N) bam_file[N] fragment_size[N] total_reads[N] `bam_file`, `fragment_size` and `total_reads` arrays should have the identical order. |
![]() Path: tools/heatmap-prepare.cwl Branch/Commit ID: 564156a9e1cc7c3679a926c479ba3ae133b1bfd4 |
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RNA-Seq pipeline single-read strand specific
Note: should be updated The original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for **strand specific single-read** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the single-read RNA-Seq data. It performs the following steps: 1. Use STAR to align reads from input FASTQ file according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 2. Use fastx_quality_stats to analyze input FASTQ file and generate quality statistics file 3. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ file to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
![]() Path: workflows/rnaseq-se-dutp.cwl Branch/Commit ID: 564156a9e1cc7c3679a926c479ba3ae133b1bfd4 |
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THOR - differential peak calling of ChIP-seq signals with replicates
What is THOR? -------------- THOR is an HMM-based approach to detect and analyze differential peaks in two sets of ChIP-seq data from distinct biological conditions with replicates. THOR performs genomic signal processing, peak calling and p-value calculation in an integrated framework. For more information please refer to: ------------------------------------- Allhoff, M., Sere K., Freitas, J., Zenke, M., Costa, I.G. (2016), Differential Peak Calling of ChIP-seq Signals with Replicates with THOR, Nucleic Acids Research, epub gkw680. |
![]() Path: workflows/rgt-thor.cwl Branch/Commit ID: 935a78f1aff757f977de4e3672aefead3b23606b |
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Single-cell Differential Expression
Single-cell Differential Expression =================================== Runs differential expression analysis for a subset of cells between two selected conditions. |
![]() Path: workflows/sc_diff_expr.cwl Branch/Commit ID: 1a46cb0e8f973481fe5ae3ae6188a41622c8532e |
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heatmap-prepare.cwl
Workflow runs homer-make-tag-directory.cwl tool using scatter for the following inputs - bam_file - fragment_size - total_reads `dotproduct` is used as a `scatterMethod`, so one element will be taken from each array to construct each job: 1) bam_file[0] fragment_size[0] total_reads[0] 2) bam_file[1] fragment_size[1] total_reads[1] ... N) bam_file[N] fragment_size[N] total_reads[N] `bam_file`, `fragment_size` and `total_reads` arrays should have the identical order. |
![]() Path: tools/heatmap-prepare.cwl Branch/Commit ID: 1a46cb0e8f973481fe5ae3ae6188a41622c8532e |
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bact_get_kmer_reference
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![]() Path: task_types/tt_bact_get_kmer_reference.cwl Branch/Commit ID: 5282690e0f634a5f83107ba878fe62cbbb347408 |
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01-qc-pe.cwl
ChIP-seq 01 QC - reads: PE |
![]() Path: v1.0/ChIP-seq_pipeline/01-qc-pe.cwl Branch/Commit ID: 6da8cef69f3a585fc3e2f4f2f730d361cbe2e978 |
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taxonomy_check_16S
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![]() Path: task_types/tt_taxonomy_check_16S.cwl Branch/Commit ID: 1b8d71c75156a1a62bf0477d59db26010e2dcc29 |
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chipseq-se.cwl
Runs ChIP-Seq BioWardrobe basic analysis with single-end data file. |
![]() Path: workflows/chipseq-se.cwl Branch/Commit ID: 12edfc2207507e53c6b5bb21e50decb5535a12f7 |