Explore Workflows
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kmer_seq_entry_extract_wnode
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![]() Path: task_types/tt_kmer_seq_entry_extract_wnode.cwl Branch/Commit ID: 5e92165ac2c11608ab2db42fe2d66eabe72dbb40 |
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FastQC - a quality control tool for high throughput sequence data
FastQC - a quality control tool for high throughput sequence data ===================================== FastQC aims to provide a simple way to do some quality control checks on raw sequence data coming from high throughput sequencing pipelines. It provides a modular set of analyses which you can use to give a quick impression of whether your data has any problems of which you should be aware before doing any further analysis. The main functions of FastQC are: - Import of data from FastQ files (any variant) - Providing a quick overview to tell you in which areas there may be problems - Summary graphs and tables to quickly assess your data - Export of results to an HTML based permanent report - Offline operation to allow automated generation of reports without running the interactive application |
![]() Path: workflows/fastqc.cwl Branch/Commit ID: 480e99a4bb3046e0565113d9dca294e0895d3b0c |
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DiffBind - Differential Binding Analysis of ChIP-Seq Peak Data
Differential Binding Analysis of ChIP-Seq Peak Data --------------------------------------------------- DiffBind processes ChIP-Seq data enriched for genomic loci where specific protein/DNA binding occurs, including peak sets identified by ChIP-Seq peak callers and aligned sequence read datasets. It is designed to work with multiple peak sets simultaneously, representing different ChIP experiments (antibodies, transcription factor and/or histone marks, experimental conditions, replicates) as well as managing the results of multiple peak callers. For more information please refer to: ------------------------------------- Ross-Innes CS, Stark R, Teschendorff AE, Holmes KA, Ali HR, Dunning MJ, Brown GD, Gojis O, Ellis IO, Green AR, Ali S, Chin S, Palmieri C, Caldas C, Carroll JS (2012). “Differential oestrogen receptor binding is associated with clinical outcome in breast cancer.” Nature, 481, -4. |
![]() Path: workflows/diffbind.cwl Branch/Commit ID: 480e99a4bb3046e0565113d9dca294e0895d3b0c |
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tt_hmmsearch_wnode.cwl
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![]() Path: task_types/tt_hmmsearch_wnode.cwl Branch/Commit ID: 656113dcac0de7cef6cff6c688f61441ee05872a |
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atm-plev.cwl
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![]() Path: scripts/cwl_workflows/atm-mon-plev/atm-plev.cwl Branch/Commit ID: cee5c228f6085b0afd4a4c4696f0c947b1514f50 |
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cram_to_bam workflow
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![]() Path: definitions/subworkflows/cram_to_bam_and_index.cwl Branch/Commit ID: fbeea265295ae596d5a3ba563e766be0c4fc26e8 |
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Bismark Methylation - pipeline for BS-Seq data analysis
Sequence reads are first cleaned from adapters and transformed into fully bisulfite-converted forward (C->T) and reverse read (G->A conversion of the forward strand) versions, before they are aligned to similarly converted versions of the genome (also C->T and G->A converted). Sequence reads that produce a unique best alignment from the four alignment processes against the bisulfite genomes (which are running in parallel) are then compared to the normal genomic sequence and the methylation state of all cytosine positions in the read is inferred. A read is considered to align uniquely if an alignment has a unique best alignment score (as reported by the AS:i field). If a read produces several alignments with the same number of mismatches or with the same alignment score (AS:i field), a read (or a read-pair) is discarded altogether. On the next step we extract the methylation call for every single C analysed. The position of every single C will be written out to a new output file, depending on its context (CpG, CHG or CHH), whereby methylated Cs will be labelled as forward reads (+), non-methylated Cs as reverse reads (-). The output of the methylation extractor is then transformed into a bedGraph and coverage file. The bedGraph counts output is then used to generate a genome-wide cytosine report which reports the number on every single CpG (optionally every single cytosine) in the genome, irrespective of whether it was covered by any reads or not. As this type of report is informative for cytosines on both strands the output may be fairly large (~46mn CpG positions or >1.2bn total cytosine positions in the human genome). |
![]() Path: workflows/bismark-methylation-se.cwl Branch/Commit ID: 480e99a4bb3046e0565113d9dca294e0895d3b0c |
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workflow.cwl
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![]() Path: workflow.cwl Branch/Commit ID: d40663cb07f9f0f700ddd1578f9a895dd8432070 |
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worldpopulation.cwl
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![]() Path: workflow/cwl/worldpopulation.cwl Branch/Commit ID: 379334455b663b295a61a0ec057bfc080f9c7c6e |
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Varscan Workflow
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![]() Path: definitions/subworkflows/varscan_pre_and_post_processing.cwl Branch/Commit ID: 6949082038c1ad36d6e9848b97a2537aef2d3805 |