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amplicon_preprocess_workflow.cwl
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![]() Path: script/1-preproccess/amplicon_preprocess_workflow.cwl Branch/Commit ID: eccef9b8e80e1a8729e7bd54f06f895a847b024d |
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Apply filters to VCF file
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![]() Path: definitions/subworkflows/filter_vcf.cwl Branch/Commit ID: ece70ac30cd87100a70f7dc64d08fa72724e9416 |
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gathered exome alignment and somatic variant detection
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![]() Path: definitions/pipelines/somatic_exome_gathered.cwl Branch/Commit ID: 43c790e2ee6a0f3f42e40fb4d9a9005eb8de747a |
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bam-bedgraph-bigwig.cwl
Workflow converts input BAM file into bigWig and bedGraph files. Input BAM file should be sorted by coordinates (required by `bam_to_bedgraph` step). If `split` input is not provided use true by default. Default logic is implemented in `valueFrom` field of `split` input inside `bam_to_bedgraph` step to avoid possible bug in cwltool with setting default values for workflow inputs. `scale` has higher priority over the `mapped_reads_number`. The last one is used to calculate `-scale` parameter for `bedtools genomecov` (step `bam_to_bedgraph`) only in a case when input `scale` is not provided. All logic is implemented inside `bedtools-genomecov.cwl`. `bigwig_filename` defines the output name only for generated bigWig file. `bedgraph_filename` defines the output name for generated bedGraph file and can influence on generated bigWig filename in case when `bigwig_filename` is not provided. All workflow inputs and outputs don't have `format` field to avoid format incompatibility errors when workflow is used as subworkflow. |
![]() Path: tools/bam-bedgraph-bigwig.cwl Branch/Commit ID: 8049a781ac4aae579fbd3036fa0bf654532f15be |
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kmer_build_tree
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![]() Path: task_types/tt_kmer_build_tree.cwl Branch/Commit ID: a539d600357a48a558daf43fc41a89aae79f9e86 |
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GSEApy - Gene Set Enrichment Analysis in Python
GSEAPY: Gene Set Enrichment Analysis in Python ============================================== Gene Set Enrichment Analysis is a computational method that determines whether an a priori defined set of genes shows statistically significant, concordant differences between two biological states (e.g. phenotypes). GSEA requires as input an expression dataset, which contains expression profiles for multiple samples. While the software supports multiple input file formats for these datasets, the tab-delimited GCT format is the most common. The first column of the GCT file contains feature identifiers (gene ids or symbols in the case of data derived from RNA-Seq experiments). The second column contains a description of the feature; this column is ignored by GSEA and may be filled with “NA”s. Subsequent columns contain the expression values for each feature, with one sample's expression value per column. It is important to note that there are no hard and fast rules regarding how a GCT file's expression values are derived. The important point is that they are comparable to one another across features within a sample and comparable to one another across samples. Tools such as DESeq2 can be made to produce properly normalized data (normalized counts) which are compatible with GSEA. |
![]() Path: workflows/gseapy.cwl Branch/Commit ID: 8049a781ac4aae579fbd3036fa0bf654532f15be |
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Cellranger Reanalyze
Cellranger Reanalyze ==================== |
![]() Path: workflows/cellranger-reanalyze.cwl Branch/Commit ID: 8049a781ac4aae579fbd3036fa0bf654532f15be |
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umi molecular alignment workflow
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![]() Path: definitions/subworkflows/molecular_alignment.cwl Branch/Commit ID: 7638b3075863ae8172f4adaec82fb2eb8e80d3d5 |
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AltAnalyze ICGS
AltAnalyze ICGS =============== |
![]() Path: workflows/altanalyze-icgs.cwl Branch/Commit ID: 8049a781ac4aae579fbd3036fa0bf654532f15be |
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Bisulfite alignment and QC
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![]() Path: definitions/pipelines/bisulfite.cwl Branch/Commit ID: 7638b3075863ae8172f4adaec82fb2eb8e80d3d5 |