Explore Workflows
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Cellranger reanalyze - reruns secondary analysis performed on the feature-barcode matrix
Devel version of Single-Cell Cell Ranger Reanalyze ================================================== Workflow calls \"cellranger aggr\" command to rerun secondary analysis performed on the feature-barcode matrix (dimensionality reduction, clustering and visualization) using different parameter settings. As an input we use filtered feature-barcode matrices in HDF5 format from cellranger count or aggr experiments. Note, we don't pass aggregation_metadata from the upstream cellranger aggr step. Need to address this issue when needed. |
![]() Path: workflows/cellranger-reanalyze.cwl Branch/Commit ID: 7eef0294395d83ff0765fce61726a59d71126422 |
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mut.cwl
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![]() Path: tests/wf/mut.cwl Branch/Commit ID: c6cced7a2e6389d2eb43342e702677ccb7c7497c |
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wgs alignment and germline variant detection
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![]() Path: definitions/pipelines/germline_wgs_gvcf.cwl Branch/Commit ID: 1750cd5cc653f058f521b6195e3bec1e7df1a086 |
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Bisulfite alignment and QC
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![]() Path: definitions/pipelines/bisulfite.cwl Branch/Commit ID: ecac0fda44df3a8f25ddfbb3e7a023fcbe4cbd0f |
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chipseq-pe.cwl
Runs ChIP-Seq BioWardrobe basic analysis with paired-end input data files. |
![]() Path: workflows/chipseq-pe.cwl Branch/Commit ID: bc75349ad3a7bdce82b4cd8584501f4d0280bb8d |
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scatter-valuefrom-wf1.cwl
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![]() Path: tests/scatter-valuefrom-wf1.cwl Branch/Commit ID: a0f2d38e37ff51721fdeaf993bb2ab474b17246b |
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gathered exome alignment and somatic variant detection
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![]() Path: definitions/pipelines/somatic_exome_gathered.cwl Branch/Commit ID: 457e101e3fb87e7fd792357afce00ed8ccbfbcdb |
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Trim Galore RNA-Seq pipeline single-read strand specific
Note: should be updated The original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for a **single-end** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the single-end RNA-Seq data. It performs the following steps: 1. Trim adapters from input FASTQ file 2. Use STAR to align reads from input FASTQ file according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 3. Use fastx_quality_stats to analyze input FASTQ file and generate quality statistics file 4. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ file to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
![]() Path: workflows/trim-rnaseq-se-dutp.cwl Branch/Commit ID: 7eef0294395d83ff0765fce61726a59d71126422 |
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basename-fields-test.cwl
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![]() Path: cwltool/schemas/v1.0/v1.0/basename-fields-test.cwl Branch/Commit ID: 62ae25772a8e98b6591554882daa3f3758079fca |
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sec-wf.cwl
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![]() Path: tests/wf/sec-wf.cwl Branch/Commit ID: e62a8406b448220969ee172699f61c5ca379d60c |