Explore Workflows
View already parsed workflows here or click here to add your own
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EMG QC workflow, (paired end version). Benchmarking with MG-RAST expt.
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Path: workflows/emg-qc-single.cwl Branch/Commit ID: 7bb76f33bf40b5cd2604001cac46f967a209c47f |
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WGS and MT analysis for fastq files
rna / protein - qc, preprocess, filter, annotation, index, abundance |
Path: CWL/Workflows/wgs-fasta.workflow.cwl Branch/Commit ID: 963ca6a73a9f8879d57c08fa59548f98f28e755a |
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RNA-Seq pipeline paired-end stranded mitochondrial
Slightly changed original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for **strand specific pair-end** experiment. An additional steps were added to map data to mitochondrial chromosome only and then merge the output. Experiment files in [FASTQ](http://maq.sourceforge.net/fastq.shtml) format either compressed or not can be used. Current workflow should be used only with the pair-end strand specific RNA-Seq data. It performs the following steps: 1. `STAR` to align reads from input FASTQ file according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 2. `fastx_quality_stats` to analyze input FASTQ file and generate quality statistics file 3. `samtools sort` to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ file to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using `GEEP` reads-counting utility; export results to file |
Path: workflows/rnaseq-pe-dutp-mitochondrial.cwl Branch/Commit ID: d1bef74924efcb8bfaa00987b3f148d5a192b7a9 |
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wgs alignment and tumor-only variant detection
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Path: definitions/pipelines/tumor_only_wgs.cwl Branch/Commit ID: 061d3a2fbcd8a1c39c0b38c549e528deb24a9d54 |
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Bisulfite alignment and QC
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Path: definitions/pipelines/bisulfite.cwl Branch/Commit ID: 1249b5d4e23d57ca5e3b8ad6d8e5f10ddb019f68 |
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Filter ChIP/ATAC peaks for Tag Density Profile or Motif Enrichment analyses
Filters ChIP/ATAC peaks with the neatest genes assigned for Tag Density Profile or Motif Enrichment analyses ============================================================================================================ Tool filters output from any ChIP/ATAC pipeline to create a file with regions of interest for Tag Density Profile or Motif Enrichment analyses. Peaks with duplicated coordinates are discarded. |
Path: workflows/filter-peaks-for-heatmap.cwl Branch/Commit ID: a1f6ca50fcb0881781b3ba0306dd61ebf555eaba |
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wgs alignment and germline variant detection
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Path: definitions/pipelines/germline_wgs.cwl Branch/Commit ID: 061d3a2fbcd8a1c39c0b38c549e528deb24a9d54 |
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Cut-n-Run pipeline paired-end
Experimental pipeline for Cut-n-Run analysis. Uses mapping results from the following experiment types: - `chipseq-pe.cwl` - `trim-chipseq-pe.cwl` - `trim-atacseq-pe.cwl` Note, the upstream analyses should not have duplicates removed |
Path: workflows/trim-chipseq-pe-cut-n-run.cwl Branch/Commit ID: 9d5cbdd3ea0bb417518115d8092584254598a440 |
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Motif Finding with HOMER with custom background regions
Motif Finding with HOMER with custom background regions --------------------------------------------------- HOMER contains a novel motif discovery algorithm that was designed for regulatory element analysis in genomics applications (DNA only, no protein). It is a differential motif discovery algorithm, which means that it takes two sets of sequences and tries to identify the regulatory elements that are specifically enriched in on set relative to the other. It uses ZOOPS scoring (zero or one occurrence per sequence) coupled with the hypergeometric enrichment calculations (or binomial) to determine motif enrichment. HOMER also tries its best to account for sequenced bias in the dataset. It was designed with ChIP-Seq and promoter analysis in mind, but can be applied to pretty much any nucleic acids motif finding problem. For more information please refer to: ------------------------------------- [Official documentation](http://homer.ucsd.edu/homer/motif/) |
Path: workflows/homer-motif-analysis-bg.cwl Branch/Commit ID: 9e3c3e65c19873cd1ed3cf7cc3b94ebc75ae0cc5 |
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kmer_cache_retrieve
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Path: task_types/tt_kmer_cache_retrieve.cwl Branch/Commit ID: a34f47d1e37af51e387ecdfa5c3047f106c1146b |
