Explore Workflows
View already parsed workflows here or click here to add your own
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js_output_workflow.cwl
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Path: tests/wf/js_output_workflow.cwl Branch/Commit ID: 6003cbb94f16103241b562f2133e7c4acac6c621 |
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js-expr-req-wf.cwl#wf
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Path: cwltool/schemas/v1.0/v1.0/js-expr-req-wf.cwl Branch/Commit ID: 26870e38cec81af880cd3e4789ae6cee8fc27020 Packed ID: wf |
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Cut-n-Run pipeline paired-end
Experimental pipeline for Cut-n-Run analysis. Uses mapping results from the following experiment types: - `chipseq-pe.cwl` - `trim-chipseq-pe.cwl` - `trim-atacseq-pe.cwl` Note, the upstream analyses should not have duplicates removed |
Path: workflows/trim-chipseq-pe-cut-n-run.cwl Branch/Commit ID: 7ae3b75bbe614e59cdeaba06047234a6c40c0fe9 |
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search.cwl#main
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Path: cwltool/schemas/v1.0/v1.0/search.cwl Branch/Commit ID: 0db44e3c9805a070564f954222efff71cd791b70 Packed ID: main |
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Trim Galore RNA-Seq pipeline paired-end
The original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for a **pair-end** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the single-end RNA-Seq data. It performs the following steps: 1. Trim adapters from input FASTQ files 2. Use STAR to align reads from input FASTQ files according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 3. Use fastx_quality_stats to analyze input FASTQ files and generate quality statistics files 4. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ files to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
Path: workflows/trim-rnaseq-pe.cwl Branch/Commit ID: bfa3843bcf36125ff258d6314f64b41336f06e6b |
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Trim Galore RNA-Seq pipeline paired-end strand specific
Modified original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for a **pair-end** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the single-end RNA-Seq data. It performs the following steps: 1. Trim adapters from input FASTQ files 2. Use STAR to align reads from input FASTQ files according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 3. Use fastx_quality_stats to analyze input FASTQ files and generate quality statistics files 4. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ files to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
Path: workflows/trim-rnaseq-pe-dutp.cwl Branch/Commit ID: 5e7385b8cfa4ddae822fff37b6bd22eb0370b389 |
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conflict.cwl#main
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Path: tests/wf/conflict.cwl Branch/Commit ID: 0e8110083bad6ea98fc487aa262953a6c5e010b5 Packed ID: main |
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Nested workflow example
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Path: tests/wf/nested.cwl Branch/Commit ID: 6003cbb94f16103241b562f2133e7c4acac6c621 |
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Generate genome index bowtie
Workflow makes indices for [bowtie](http://bowtie-bio.sourceforge.net/tutorial.shtml) v1.2.0 (12/30/2016). Executes `bowtie-index` to generate indices requires genome [FASTA](http://zhanglab.ccmb.med.umich.edu/FASTA/) file as input, returns results as a directory |
Path: workflows/bowtie-index.cwl Branch/Commit ID: bfa3843bcf36125ff258d6314f64b41336f06e6b |
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schemadef-wf.cwl
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Path: cwltool/schemas/v1.0/v1.0/schemadef-wf.cwl Branch/Commit ID: 26870e38cec81af880cd3e4789ae6cee8fc27020 |
