Explore Workflows
View already parsed workflows here or click here to add your own
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allele-process-strain.cwl
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Path: subworkflows/allele-process-strain.cwl Branch/Commit ID: 2b8146f76595f0c4d8bf692de78b21280162b1d0 |
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RNA-Seq pipeline paired-end strand specific
The original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for a **paired-end** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the paired-end RNA-Seq data. It performs the following steps: 1. Use STAR to align reads from input FASTQ files according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 2. Use fastx_quality_stats to analyze input FASTQ files and generate quality statistics files 3. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 4. Generate BigWig file on the base of sorted BAM file 5. Map input FASTQ files to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 6. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
Path: workflows/rnaseq-pe-dutp.cwl Branch/Commit ID: 581156366f91861bd4dbb5bcb59f67d468b32af3 |
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collapsed_fastq_to_bam.cwl
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Path: workflows/marianas/collapsed_fastq_to_bam.cwl Branch/Commit ID: 9998da2da694af2edad7c2135f6995e2282794a3 |
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Generate genome indices for STAR & bowtie
Creates indices for: * [STAR](https://github.com/alexdobin/STAR) v2.5.3a (03/17/2017) PMID: [23104886](https://www.ncbi.nlm.nih.gov/pubmed/23104886) * [bowtie](http://bowtie-bio.sourceforge.net/tutorial.shtml) v1.2.0 (12/30/2016) It performs the following steps: 1. `STAR --runMode genomeGenerate` to generate indices, based on [FASTA](http://zhanglab.ccmb.med.umich.edu/FASTA/) and [GTF](http://mblab.wustl.edu/GTF2.html) input files, returns results as an array of files 2. Outputs indices as [Direcotry](http://www.commonwl.org/v1.0/CommandLineTool.html#Directory) data type 3. Separates *chrNameLength.txt* file from Directory output 4. `bowtie-build` to generate indices requires genome [FASTA](http://zhanglab.ccmb.med.umich.edu/FASTA/) file as input, returns results as a group of main and secondary files |
Path: workflows/genome-indices.cwl Branch/Commit ID: 12c29f88855329192bfff977f046990031f04931 |
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advanced-header.cwl
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Path: metadata/advanced-header.cwl Branch/Commit ID: e7a896db41063c52197aec8d5b49ed013d7a5ba7 |
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kmer_cache_store
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Path: task_types/tt_kmer_cache_store.cwl Branch/Commit ID: f5c11df465aaadf712c38ba4933679fe1cbe03ca |
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group-isoforms-batch.cwl
Workflow runs group-isoforms.cwl tool using scatter for isoforms_file input. genes_filename and common_tss_filename inputs are ignored. |
Path: tools/group-isoforms-batch.cwl Branch/Commit ID: 1131f82a53315cca217a6c84b3bd272aa62e4bca |
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count-lines9-wf-noET.cwl
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Path: tests/count-lines9-wf-noET.cwl Branch/Commit ID: 86c46cb397de029e4c91f02cca40fa2b54d22f37 |
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duplicated_readgroup_fix.cwl
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Path: genomel/cwl/workflows/utils/duplicated_readgroup_fix.cwl Branch/Commit ID: 286bce04c474d28bddeb7dbe43ab8d59919fe855 |
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kmer_top_n_extract
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Path: task_types/tt_kmer_top_n_extract.cwl Branch/Commit ID: 7319ccfd2108929588bdc266d9df198629dfaa65 |
