Explore Workflows
View already parsed workflows here or click here to add your own
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blastp_wnode_naming
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Path: task_types/tt_blastp_wnode_naming.cwl Branch/Commit ID: 09774c78a965dd8f6c315597a53eef5998a3c1b6 |
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allele-vcf-alignreads-se-pe.cwl
Workflow maps FASTQ files from `fastq_files` input into reference genome `reference_star_indices_folder` and insilico generated `insilico_star_indices_folder` genome (concatenated genome for both `strain1` and `strain2` strains). For both genomes STAR is run with `outFilterMultimapNmax` parameter set to 1 to discard all of the multimapped reads. For insilico genome SAM file is generated. Then it's splitted into two SAM files based on strain names and then sorted by coordinates into the BAM format. For reference genome output BAM file from STAR slignment is also coordinate sorted. |
Path: subworkflows/allele-vcf-alignreads-se-pe.cwl Branch/Commit ID: e284e3f6dff25037b209895c52f2abd37a1ce1bf |
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trim-rnaseq-pe.cwl
Runs RNA-Seq BioWardrobe basic analysis with pair-end data file. |
Path: workflows/trim-rnaseq-pe.cwl Branch/Commit ID: e706ffe742cfdf713c4315ab2fb56d07f7e688cb |
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Xenbase ChIP-Seq pipeline single-read
1. Convert input SRA file into FASTQ file (run fastq-dump) 2. Analyze quality of FASTQ file (run fastqc) 3. If any of the following fields in fastqc generated report is marked as failed: \"Per base sequence quality\", \"Per sequence quality scores\", \"Overrepresented sequences\", \"Adapter Content\", - trim adapters (run trimmomatic) 4. Align original or trimmed FASTQ file to reference genome (run Bowtie2) 5. Sort and index generated by Bowtie2 BAM file (run samtools sort, samtools index) 6. Remove duplicates in sorted BAM file (run picard) 7. Sort and index BAM file after duplicates removing (run samtools sort, samtools index) 8. Count mapped reads number in sorted BAM file (run bamtools stats) 9. Generate genome coverage BED file (run bedtools genomecov) 10. Sort genearted BED file (run sort) 11. Generate genome coverage bigWig file from BED file (run bedGraphToBigWig) |
Path: workflows/xenbase-chipseq-se.cwl Branch/Commit ID: e238d1756f1db35571e84d72e1699e5d1540f10c |
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io-int-default-wf.cwl
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Path: v1.0/v1.0/io-int-default-wf.cwl Branch/Commit ID: a5ae5ad0c9017ed625fb372f65e72dbb069439b0 |
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RNA-Seq pipeline paired-end
The original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for a **paired-end** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the paired-end RNA-Seq data. It performs the following steps: 1. Use STAR to align reads from input FASTQ files according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 2. Use fastx_quality_stats to analyze input FASTQ files and generate quality statistics files 3. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 4. Generate BigWig file on the base of sorted BAM file 5. Map input FASTQ files to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 6. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
Path: workflows/rnaseq-pe.cwl Branch/Commit ID: 2b8146f76595f0c4d8bf692de78b21280162b1d0 |
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Trim Galore RNA-Seq pipeline paired-end
The original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for a **pair-end** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the single-end RNA-Seq data. It performs the following steps: 1. Trim adapters from input FASTQ files 2. Use STAR to align reads from input FASTQ files according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 3. Use fastx_quality_stats to analyze input FASTQ files and generate quality statistics files 4. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ files to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
Path: workflows/trim-rnaseq-pe.cwl Branch/Commit ID: e238d1756f1db35571e84d72e1699e5d1540f10c |
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tt_blastn_wnode
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Path: task_types/tt_blastn_wnode.cwl Branch/Commit ID: 6a29751f2b16659c1592f1e94837c989e68f3b8b |
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xenbase-sra-to-fastq-se.cwl
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Path: subworkflows/xenbase-sra-to-fastq-se.cwl Branch/Commit ID: 3b2e0de49d9ee6fd9a8c9580b6a02d0f7e4c8f7c |
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screen out taxa
Remove sequences which align against a reference set using bowtie2. The references are preformatted (index files) |
Path: CWL/Workflows/organism-screening.workflow.cwl Branch/Commit ID: 3e967f035c10a176b9457331df0b3374a8562b26 |
