Explore Workflows
View already parsed workflows here or click here to add your own
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HLA_LA_workflow_full.cwl
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![]() Path: HLA_LA_workflow_full.cwl Branch/Commit ID: f6d1debfd7fcaf203a38aad7ff57f689c2cbbbfd |
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Filter single sample sv vcf from depth callers(cnvkit/cnvnator)
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![]() Path: definitions/subworkflows/sv_depth_caller_filter.cwl Branch/Commit ID: 5be54bf09092c53e6c7797a875f64a360d511d7f |
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Bismark Methylation - pipeline for BS-Seq data analysis
Sequence reads are first cleaned from adapters and transformed into fully bisulfite-converted forward (C->T) and reverse read (G->A conversion of the forward strand) versions, before they are aligned to similarly converted versions of the genome (also C->T and G->A converted). Sequence reads that produce a unique best alignment from the four alignment processes against the bisulfite genomes (which are running in parallel) are then compared to the normal genomic sequence and the methylation state of all cytosine positions in the read is inferred. A read is considered to align uniquely if an alignment has a unique best alignment score (as reported by the AS:i field). If a read produces several alignments with the same number of mismatches or with the same alignment score (AS:i field), a read (or a read-pair) is discarded altogether. On the next step we extract the methylation call for every single C analysed. The position of every single C will be written out to a new output file, depending on its context (CpG, CHG or CHH), whereby methylated Cs will be labelled as forward reads (+), non-methylated Cs as reverse reads (-). The output of the methylation extractor is then transformed into a bedGraph and coverage file. The bedGraph counts output is then used to generate a genome-wide cytosine report which reports the number on every single CpG (optionally every single cytosine) in the genome, irrespective of whether it was covered by any reads or not. As this type of report is informative for cytosines on both strands the output may be fairly large (~46mn CpG positions or >1.2bn total cytosine positions in the human genome). |
![]() Path: workflows/bismark-methylation-se.cwl Branch/Commit ID: 2c486543c335bb99b245dfe7e2f033f535efb9cf |
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bam-bedgraph-bigwig.cwl
Workflow converts input BAM file into bigWig and bedGraph files. Input BAM file should be sorted by coordinates (required by `bam_to_bedgraph` step). If `split` input is not provided use true by default. Default logic is implemented in `valueFrom` field of `split` input inside `bam_to_bedgraph` step to avoid possible bug in cwltool with setting default values for workflow inputs. `scale` has higher priority over the `mapped_reads_number`. The last one is used to calculate `-scale` parameter for `bedtools genomecov` (step `bam_to_bedgraph`) only in a case when input `scale` is not provided. All logic is implemented inside `bedtools-genomecov.cwl`. `bigwig_filename` defines the output name only for generated bigWig file. `bedgraph_filename` defines the output name for generated bedGraph file and can influence on generated bigWig filename in case when `bigwig_filename` is not provided. All workflow inputs and outputs don't have `format` field to avoid format incompatibility errors when workflow is used as subworkflow. |
![]() Path: tools/bam-bedgraph-bigwig.cwl Branch/Commit ID: a1f6ca50fcb0881781b3ba0306dd61ebf555eaba |
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waltz-workflow.cwl
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![]() Path: workflows/waltz/waltz-workflow.cwl Branch/Commit ID: 0bd60a8962cc9960b7e4f6528547e220bcd2b941 |
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group-isoforms-batch.cwl
Workflow runs group-isoforms.cwl tool using scatter for isoforms_file input. genes_filename and common_tss_filename inputs are ignored. |
![]() Path: tools/group-isoforms-batch.cwl Branch/Commit ID: 5c0d21fe4f180730d4f6dd301e785bd6b00d5907 |
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qc_workflow_wo_waltz.cwl
This workflow is intended to be used to test the QC module, without having to run the long waltz step |
![]() Path: workflows/QC/qc_workflow_wo_waltz.cwl Branch/Commit ID: daba08457dd53fb81d11acb274c29a77b6122316 |
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Build Bowtie indices
Workflow runs [Bowtie](http://bowtie-bio.sourceforge.net/tutorial.shtml) v1.2.0 (12/30/2016) to build indices for reference genome provided in a single FASTA file as fasta_file input. Generated indices are saved in a folder with the name that corresponds to the input genome |
![]() Path: workflows/bowtie-index.cwl Branch/Commit ID: 2c486543c335bb99b245dfe7e2f033f535efb9cf |
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HS Metrics workflow
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![]() Path: definitions/subworkflows/hs_metrics.cwl Branch/Commit ID: d57c2af01a3cb6016e5a264f60641eafd2e5aa05 |
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search.cwl#main
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![]() Path: cwltool/schemas/v1.0/v1.0/search.cwl Branch/Commit ID: 89ccbfc53ff3bb6abe2eb90bb7e0091c54c18f5c Packed ID: main |