Explore Workflows
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js-expr-req-wf.cwl#wf
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![]() Path: cwltool/schemas/v1.0/v1.0/js-expr-req-wf.cwl Branch/Commit ID: c7c379948c02ba8f048d157f06eb903b1bda9894 Packed ID: wf |
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js-expr-req-wf.cwl#wf
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![]() Path: tests/js-expr-req-wf.cwl Branch/Commit ID: 50251ef931d108c09bed2d330d3d4fe9c562b1c3 Packed ID: wf |
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Trim Galore RNA-Seq pipeline paired-end strand specific
Modified original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for a **pair-end** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the single-end RNA-Seq data. It performs the following steps: 1. Trim adapters from input FASTQ files 2. Use STAR to align reads from input FASTQ files according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 3. Use fastx_quality_stats to analyze input FASTQ files and generate quality statistics files 4. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ files to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
![]() Path: workflows/trim-rnaseq-pe-dutp.cwl Branch/Commit ID: 10ce6e113f749c7bd725e426445220c3bdc5ddf1 |
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harmonization_bwa_mem.cwl
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![]() Path: genomel/cwl/workflows/harmonization/harmonization_bwa_mem.cwl Branch/Commit ID: 7eb6b9f75db20866611a3bc55bee28746e84c1b6 |
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exome alignment and germline variant detection, with optitype for HLA typing
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![]() Path: definitions/pipelines/germline_exome_hla_typing.cwl Branch/Commit ID: f77a920bcc73f6cfdb091eed75a149d02cd8a263 |
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Pairwise genomic regions intersection
Pairwise genomic regions intersection ============================================= Overlaps peaks from two ChIP/ATAC experiments |
![]() Path: workflows/peak-intersect.cwl Branch/Commit ID: 2005c6b7f1bff6247d015ff6c116bd9ec97158bb |
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count-lines6-wf.cwl
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![]() Path: cwltool/schemas/v1.0/v1.0/count-lines6-wf.cwl Branch/Commit ID: 814bd0405a7701efc7d63e8f0179df394c7766f7 |
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conflict-wf.cwl#collision
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![]() Path: cwltool/schemas/v1.0/v1.0/conflict-wf.cwl Branch/Commit ID: fec7a10466a26e376b14181a88734983cfb1b8cb Packed ID: collision |
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Nested workflow example
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![]() Path: tests/wf/nested.cwl Branch/Commit ID: 4642316a30a95d4f3d135c18f98477886b160094 |
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scatter-valuefrom-wf2.cwl
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![]() Path: cwltool/schemas/v1.0/v1.0/scatter-valuefrom-wf2.cwl Branch/Commit ID: cf9d7cdefa6dfb3b678636da02bc55b6108c04ac |