Explore Workflows
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RNA-Seq pipeline paired-end
The original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for a **paired-end** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the paired-end RNA-Seq data. It performs the following steps: 1. Use STAR to align reads from input FASTQ files according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 2. Use fastx_quality_stats to analyze input FASTQ files and generate quality statistics files 3. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 4. Generate BigWig file on the base of sorted BAM file 5. Map input FASTQ files to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 6. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
![]() Path: workflows/rnaseq-pe.cwl Branch/Commit ID: 10ce6e113f749c7bd725e426445220c3bdc5ddf1 |
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WGS QC workflow
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![]() Path: definitions/subworkflows/qc_wgs.cwl Branch/Commit ID: 1750cd5cc653f058f521b6195e3bec1e7df1a086 |
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align_sort_sa
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![]() Path: task_types/tt_align_sort_sa.cwl Branch/Commit ID: a34f47d1e37af51e387ecdfa5c3047f106c1146b |
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Genomic regions intersection and visualization
Genomic regions intersection and visualization ============================================== 1. Merges intervals within each of the filtered peaks files from ChIP/ATAC experiments 2. Overlaps merged intervals and assigns the nearest genes to them |
![]() Path: workflows/intervene.cwl Branch/Commit ID: 581156366f91861bd4dbb5bcb59f67d468b32af3 |
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EMG pipeline v4.0 (single end version)
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![]() Path: workflows/emg-pipeline-v4-single.cwl Branch/Commit ID: cac44f2cf14110fde9951161c663c4525772f616 |
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Subworkflow that runs cnvkit in single sample mode and returns a vcf file
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![]() Path: definitions/subworkflows/cnvkit_single_sample.cwl Branch/Commit ID: a7838a5ca72b25db5c2af20a15f34303a839980e |
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Get Proteins
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![]() Path: wf_bacterial_prot_src.cwl Branch/Commit ID: 22ffe27d9d4a899def7592d75d5871c1856adbdb |
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format_rrnas_from_seq_entry
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![]() Path: task_types/tt_format_rrnas_from_seq_entry.cwl Branch/Commit ID: 7319ccfd2108929588bdc266d9df198629dfaa65 |
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harmonization_novoalign_multi_readgroup.cwl
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![]() Path: genomel/cwl/workflows/harmonization/harmonization_novoalign_multi_readgroup.cwl Branch/Commit ID: 3c62f1ddf8a54601da3cfc16c37d0cb0d6a2ea28 |
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conflict-wf.cwl#collision
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![]() Path: cwltool/schemas/v1.0/v1.0/conflict-wf.cwl Branch/Commit ID: 9e7c68c0834645ba53a7e2b5f70d53df9d051c92 Packed ID: collision |