Explore Workflows
View already parsed workflows here or click here to add your own
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adapter for sequence_align_and_tag
Some workflow engines won't stage files in our nested structure, so parse it out here |
![]() Path: definitions/subworkflows/sequence_align_and_tag_adapter.cwl Branch/Commit ID: a7838a5ca72b25db5c2af20a15f34303a839980e |
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assm_assm_blastn_wnode
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![]() Path: task_types/tt_assm_assm_blastn_wnode.cwl Branch/Commit ID: f5c11df465aaadf712c38ba4933679fe1cbe03ca |
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kmer_seq_entry_extract_wnode
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![]() Path: task_types/tt_kmer_seq_entry_extract_wnode.cwl Branch/Commit ID: bba6c580ab88e077f6aa2c2ee7c73159f3f9156e |
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Build STAR indices
Workflow runs [STAR](https://github.com/alexdobin/STAR) v2.5.3a (03/17/2017) PMID: [23104886](https://www.ncbi.nlm.nih.gov/pubmed/23104886) to build indices for reference genome provided in a single FASTA file as fasta_file input and GTF annotation file from annotation_gtf_file input. Generated indices are saved in a folder with the name that corresponds to the input genome. |
![]() Path: workflows/star-index.cwl Branch/Commit ID: 10ce6e113f749c7bd725e426445220c3bdc5ddf1 |
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Conversion and compression of RDF files
Workflow to convert a RDF file to the HDT format and GZIP compress it for long term storage |
![]() Path: cwl/workflows/workflow_toHDT_compression.cwl Branch/Commit ID: b9097b82e6ab6f2c9496013ce4dd6877092956a0 |
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running cellranger mkfastq and count
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![]() Path: definitions/subworkflows/cellranger_mkfastq_and_count.cwl Branch/Commit ID: 1750cd5cc653f058f521b6195e3bec1e7df1a086 |
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RNA-Seq pipeline single-read
The original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for a **single-read** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the single-read RNA-Seq data. It performs the following steps: 1. Use STAR to align reads from input FASTQ file according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 2. Use fastx_quality_stats to analyze input FASTQ file and generate quality statistics file 3. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ file to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
![]() Path: workflows/rnaseq-se.cwl Branch/Commit ID: 1131f82a53315cca217a6c84b3bd272aa62e4bca |
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tt_hmmsearch_wnode.cwl
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![]() Path: task_types/tt_hmmsearch_wnode.cwl Branch/Commit ID: 146df33e2e44afa2a608ac72c036e6b6b871af93 |
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Bismark Methylation - pipeline for BS-Seq data analysis
Sequence reads are first cleaned from adapters and transformed into fully bisulfite-converted forward (C->T) and reverse read (G->A conversion of the forward strand) versions, before they are aligned to similarly converted versions of the genome (also C->T and G->A converted). Sequence reads that produce a unique best alignment from the four alignment processes against the bisulfite genomes (which are running in parallel) are then compared to the normal genomic sequence and the methylation state of all cytosine positions in the read is inferred. A read is considered to align uniquely if an alignment has a unique best alignment score (as reported by the AS:i field). If a read produces several alignments with the same number of mismatches or with the same alignment score (AS:i field), a read (or a read-pair) is discarded altogether. On the next step we extract the methylation call for every single C analysed. The position of every single C will be written out to a new output file, depending on its context (CpG, CHG or CHH), whereby methylated Cs will be labelled as forward reads (+), non-methylated Cs as reverse reads (-). The output of the methylation extractor is then transformed into a bedGraph and coverage file. The bedGraph counts output is then used to generate a genome-wide cytosine report which reports the number on every single CpG (optionally every single cytosine) in the genome, irrespective of whether it was covered by any reads or not. As this type of report is informative for cytosines on both strands the output may be fairly large (~46mn CpG positions or >1.2bn total cytosine positions in the human genome). |
![]() Path: workflows/bismark-methylation-se.cwl Branch/Commit ID: 1131f82a53315cca217a6c84b3bd272aa62e4bca |
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host.sort.workflow.cwl
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![]() Path: host.sort.workflow.cwl Branch/Commit ID: d79d09f92c64e25d1a68af7e8aed5e48e73e6537 |