Explore Workflows
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Feature expression merge - combines feature expression from several experiments
Feature expression merge - combines feature expression from several experiments ========================================================================= Workflows merges RPKM (by default) gene expression from several experiments based on the values from GeneId, Chrom, TxStart, TxEnd and Strand columns (by default). Reported unique columns are renamed based on the experiments names. |
![]() Path: workflows/feature-merge.cwl Branch/Commit ID: 1131f82a53315cca217a6c84b3bd272aa62e4bca |
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FASTQ to BQSR
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![]() Path: definitions/subworkflows/fastq_to_bqsr.cwl Branch/Commit ID: 295e7b7f51727c0f2d6cc86ce817449b2e8dba3c |
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bam to trimmed fastqs and biscuit alignments
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![]() Path: definitions/subworkflows/bam_to_trimmed_fastq_and_biscuit_alignments.cwl Branch/Commit ID: 1249b5d4e23d57ca5e3b8ad6d8e5f10ddb019f68 |
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Gathered Downsample and HaplotypeCaller
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![]() Path: definitions/pipelines/gathered_downsample_and_recall.cwl Branch/Commit ID: 049f4aeff4c4a1b8421cac9b1c1c1f0da5848315 |
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transcriptome_assemble.cwl
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![]() Path: workflow/transcriptome_assemble/transcriptome_assemble.cwl Branch/Commit ID: 4c90bc270e271b24caacaf000116727ff8ec81f8 |
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umi molecular alignment workflow
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![]() Path: definitions/subworkflows/molecular_alignment.cwl Branch/Commit ID: 441b85003fdc10cf4cbf333d89acb4d23b0fef32 |
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bird_scatter_values.cwl
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![]() Path: 03_bird_scatter_values/bird_scatter_values.cwl Branch/Commit ID: 5e08b9b8a0323a1f4740a65bdb356e9b75074093 |
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FastQC - a quality control tool for high throughput sequence data
FastQC - a quality control tool for high throughput sequence data ===================================== FastQC aims to provide a simple way to do some quality control checks on raw sequence data coming from high throughput sequencing pipelines. It provides a modular set of analyses which you can use to give a quick impression of whether your data has any problems of which you should be aware before doing any further analysis. The main functions of FastQC are: - Import of data from FastQ files (any variant) - Providing a quick overview to tell you in which areas there may be problems - Summary graphs and tables to quickly assess your data - Export of results to an HTML based permanent report - Offline operation to allow automated generation of reports without running the interactive application |
![]() Path: workflows/fastqc.cwl Branch/Commit ID: ee66d03be8a7fd61367db40c37a973ff55ece4da |
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RNA-Seq alignment and transcript/gene abundance workflow
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![]() Path: definitions/pipelines/rnaseq.cwl Branch/Commit ID: 049f4aeff4c4a1b8421cac9b1c1c1f0da5848315 |
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MAnorm SE - quantitative comparison of ChIP-Seq single-read data
What is MAnorm? -------------- MAnorm is a robust model for quantitative comparison of ChIP-Seq data sets of TFs (transcription factors) or epigenetic modifications and you can use it for: * Normalization of two ChIP-seq samples * Quantitative comparison (differential analysis) of two ChIP-seq samples * Evaluating the overlap enrichment of the protein binding sites(peaks) * Elucidating underlying mechanisms of cell-type specific gene regulation How MAnorm works? ---------------- MAnorm uses common peaks of two samples as a reference to build the rescaling model for normalization, which is based on the empirical assumption that if a chromatin-associated protein has a substantial number of peaks shared in two conditions, the binding at these common regions will tend to be determined by similar mechanisms, and thus should exhibit similar global binding intensities across samples. The observed differences on common peaks are presumed to reflect the scaling relationship of ChIP-Seq signals between two samples, which can be applied to all peaks. What do the inputs mean? ---------------- ### General **Experiment short name/Alias** * short name for you experiment to identify among the others **ChIP-Seq SE sample 1** * previously analyzed ChIP-Seq single-read experiment to be used as Sample 1 **ChIP-Seq SE sample 2** * previously analyzed ChIP-Seq single-read experiment to be used as Sample 2 **Genome** * Reference genome to be used for gene assigning ### Advanced **Reads shift size for sample 1** * This value is used to shift reads towards 3' direction to determine the precise binding site. Set as half of the fragment length. Default 100 **Reads shift size for sample 2** * This value is used to shift reads towards 5' direction to determine the precise binding site. Set as half of the fragment length. Default 100 **M-value (log2-ratio) cutoff** * Absolute M-value (log2-ratio) cutoff to define biased (differential binding) peaks. Default: 1.0 **P-value cutoff** * P-value cutoff to define biased peaks. Default: 0.01 **Window size** * Window size to count reads and calculate read densities. 2000 is recommended for sharp histone marks like H3K4me3 and H3K27ac, and 1000 for TFs or DNase-seq. Default: 2000 |
![]() Path: workflows/manorm-se.cwl Branch/Commit ID: 1131f82a53315cca217a6c84b3bd272aa62e4bca |