Explore Workflows
View already parsed workflows here or click here to add your own
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Run genomic CMsearch
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Path: bacterial_noncoding/wf_gcmsearch.cwl Branch/Commit ID: 4ffbad9ffeab15ec8af5f6f91bd352ef96d1ef77 |
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io-int-optional-wf.cwl
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Path: tests/io-int-optional-wf.cwl Branch/Commit ID: 3e90671b25f7840ef2926ad2bacbf447772dda94 |
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echo-wf-default.cwl
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Path: tests/echo-wf-default.cwl Branch/Commit ID: 3e90671b25f7840ef2926ad2bacbf447772dda94 |
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Kallisto index pipeline
This workflow indexes the input reference FASTA with kallisto, and generates a kallisto index file (.kdx). This index sample can then be used as input into the kallisto transcript-level quantification workflow (kallisto-quant-pe.cwl), or others that may include this workflow as an upstream source. ### __Inputs__ - FASTA file of the reference genome that will be indexed - number of threads to use for multithreading processes ### __Outputs__ - kallisto index file (.kdx). - stdout log file (output in Overview tab as well) - stderr log file ### __Data Analysis Steps__ 1. cwl calls dockercontainer robertplayer/scidap-kallisto to index reference FASTA with `kallisto index`, generating a kallisto index file. ### __References__ - Bray, N. L., Pimentel, H., Melsted, P. & Pachter, L. Near-optimal probabilistic RNA-seq quantification, Nature Biotechnology 34, 525-527(2016), doi:10.1038/nbt.3519 |
Path: workflows/kallisto-index.cwl Branch/Commit ID: d76110e0bfc40c874f82e37cef6451d74df4f908 |
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extract_gencoll_ids
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Path: task_types/tt_extract_gencoll_ids.cwl Branch/Commit ID: 5b498b4c4f17bb8f17e6886aa4c5661d7aba34fc |
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conflict-wf.cwl#collision
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Path: tests/conflict-wf.cwl Branch/Commit ID: 7d7986a6e852ca6e3239c96d3a05dd536c76c903 Packed ID: collision |
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variant-calling-pair.cwl
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Path: modules/pair/variant-calling-pair.cwl Branch/Commit ID: 7c694b51d9d593439cf8ab3e5006665f0bbe2149 |
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tt_kmer_top_n.cwl
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Path: task_types/tt_kmer_top_n.cwl Branch/Commit ID: 1cfd46014be8d867044cb10d1ddde0cb3068ee84 |
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wf-loadContents4.cwl
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Path: tests/wf-loadContents4.cwl Branch/Commit ID: 3e90671b25f7840ef2926ad2bacbf447772dda94 |
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Trim Galore RNA-Seq pipeline paired-end
The original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for a **pair-end** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the single-end RNA-Seq data. It performs the following steps: 1. Trim adapters from input FASTQ files 2. Use STAR to align reads from input FASTQ files according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 3. Use fastx_quality_stats to analyze input FASTQ files and generate quality statistics files 4. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ files to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
Path: workflows/trim-rnaseq-pe.cwl Branch/Commit ID: 8a92669a566589d80fde9d151054ffc220ed4ddd |
