Explore Workflows
View already parsed workflows here or click here to add your own
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count-lines11-wf-noET.cwl
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Path: tests/count-lines11-wf-noET.cwl Branch/Commit ID: 707ebcd2173889604459c5f4ffb55173c508abb3 |
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Motif Finding with HOMER with custom background regions
Motif Finding with HOMER with custom background regions --------------------------------------------------- HOMER contains a novel motif discovery algorithm that was designed for regulatory element analysis in genomics applications (DNA only, no protein). It is a differential motif discovery algorithm, which means that it takes two sets of sequences and tries to identify the regulatory elements that are specifically enriched in on set relative to the other. It uses ZOOPS scoring (zero or one occurrence per sequence) coupled with the hypergeometric enrichment calculations (or binomial) to determine motif enrichment. HOMER also tries its best to account for sequenced bias in the dataset. It was designed with ChIP-Seq and promoter analysis in mind, but can be applied to pretty much any nucleic acids motif finding problem. For more information please refer to: ------------------------------------- [Official documentation](http://homer.ucsd.edu/homer/motif/) |
Path: workflows/homer-motif-analysis-bg.cwl Branch/Commit ID: 261c0232a7a40880f2480b811ed2d7e89c463869 |
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foreign_screening.cwl
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Path: vecscreen/foreign_screening.cwl Branch/Commit ID: 5b498b4c4f17bb8f17e6886aa4c5661d7aba34fc |
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Build Bowtie indices
Workflow runs [Bowtie](http://bowtie-bio.sourceforge.net/tutorial.shtml) v1.2.0 (12/30/2016) to build indices for reference genome provided in a single FASTA file as fasta_file input. Generated indices are saved in a folder with the name that corresponds to the input genome |
Path: workflows/bowtie-index.cwl Branch/Commit ID: 261c0232a7a40880f2480b811ed2d7e89c463869 |
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SoupX (workflow) - an R package for the estimation and removal of cell free mRNA contamination
Wrapped in a workflow SoupX tool for easy access to Cell Ranger pipeline compressed outputs. |
Path: tools/soupx-subworkflow.cwl Branch/Commit ID: 261c0232a7a40880f2480b811ed2d7e89c463869 |
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Single-Cell ATAC-Seq Dimensionality Reduction Analysis
Single-Cell ATAC-Seq Dimensionality Reduction Analysis Removes noise and confounding sources of variation by reducing dimensionality of chromatin accessibility data from the outputs of “Single-Cell Multiome ATAC and RNA-Seq Filtering Analysis” pipelines. The results of this workflow are primarily used in “Single-Cell ATAC-Seq Cluster Analysis” or “Single-Cell WNN Cluster Analysis” pipelines. |
Path: workflows/sc-atac-reduce.cwl Branch/Commit ID: 261c0232a7a40880f2480b811ed2d7e89c463869 |
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umi per-lane alignment subworkflow
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Path: definitions/subworkflows/umi_alignment.cwl Branch/Commit ID: ddb49a0951d9ad537269d7db3fe8f904495a8bf4 |
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Single-cell Format Transform
Single-cell Format Transform Transforms single-cell sequencing data formats into Cell Ranger like output. |
Path: workflows/sc-format-transform.cwl Branch/Commit ID: 261c0232a7a40880f2480b811ed2d7e89c463869 |
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Trim Galore RNA-Seq pipeline single-read strand specific
Note: should be updated The original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for a **single-end** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the single-end RNA-Seq data. It performs the following steps: 1. Trim adapters from input FASTQ file 2. Use STAR to align reads from input FASTQ file according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 3. Use fastx_quality_stats to analyze input FASTQ file and generate quality statistics file 4. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ file to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
Path: workflows/trim-rnaseq-se-dutp.cwl Branch/Commit ID: b957a4f681bf0ca8ebba4e0d0ec3936bf79620c5 |
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heatmap-prepare.cwl
Workflow runs homer-make-tag-directory.cwl tool using scatter for the following inputs - bam_file - fragment_size - total_reads `dotproduct` is used as a `scatterMethod`, so one element will be taken from each array to construct each job: 1) bam_file[0] fragment_size[0] total_reads[0] 2) bam_file[1] fragment_size[1] total_reads[1] ... N) bam_file[N] fragment_size[N] total_reads[N] `bam_file`, `fragment_size` and `total_reads` arrays should have the identical order. |
Path: subworkflows/heatmap-prepare.cwl Branch/Commit ID: e284e3f6dff25037b209895c52f2abd37a1ce1bf |
