Explore Workflows
View already parsed workflows here or click here to add your own
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allele-alignreads-se-pe.cwl
Workflow maps FASTQ files from `fastq_files` input into reference genome `reference_star_indices_folder` and insilico generated `insilico_star_indices_folder` genome (concatenated genome for both `strain1` and `strain2` strains). For both genomes STAR is run with `outFilterMultimapNmax` parameter set to 1 to discard all of the multimapped reads. For insilico genome SAM file is generated. Then it's splitted into two SAM files based on strain names and then sorted by coordinates into the BAM format. For reference genome output BAM file from STAR slignment is also coordinate sorted. |
Path: subworkflows/allele-alignreads-se-pe.cwl Branch/Commit ID: b25b17651171f32005e9d879a9a049382f044baf |
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merge and annotate svs with population allele freq and vep
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Path: definitions/subworkflows/merge_svs.cwl Branch/Commit ID: ad65dc1dfff9afa5077f498b85e699716c47f6cb |
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Unaligned bam to sorted, markduped bam
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Path: definitions/subworkflows/align_sort_markdup.cwl Branch/Commit ID: d57c2af01a3cb6016e5a264f60641eafd2e5aa05 |
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Build STAR indices
Workflow runs [STAR](https://github.com/alexdobin/STAR) v2.5.3a (03/17/2017) PMID: [23104886](https://www.ncbi.nlm.nih.gov/pubmed/23104886) to build indices for reference genome provided in a single FASTA file as fasta_file input and GTF annotation file from annotation_gtf_file input. Generated indices are saved in a folder with the name that corresponds to the input genome. |
Path: workflows/star-index.cwl Branch/Commit ID: 42dc4f70b117e78785b82865ec4c4b941ac1c259 |
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tmb_workflow.cwl
Workflow to run the TMB analysis on a batch of samples and merge the results back into a single data clinical file |
Path: cwl/tmb_workflow.cwl Branch/Commit ID: 5cad957fec135aa55ca8d588372db0557ca1cad5 |
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cluster_blastp_wnode and gpx_qdump combined
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Path: task_types/tt_cluster_and_qdump.cwl Branch/Commit ID: ae781871782805632f8947c1b11f65507c80cd43 |
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RNA-Seq pipeline paired-end
The original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for a **paired-end** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the paired-end RNA-Seq data. It performs the following steps: 1. Use STAR to align reads from input FASTQ files according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 2. Use fastx_quality_stats to analyze input FASTQ files and generate quality statistics files 3. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 4. Generate BigWig file on the base of sorted BAM file 5. Map input FASTQ files to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 6. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
Path: workflows/rnaseq-pe.cwl Branch/Commit ID: 42dc4f70b117e78785b82865ec4c4b941ac1c259 |
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count-lines9-wf.cwl
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Path: tests/count-lines9-wf.cwl Branch/Commit ID: 57baec040c99d7edef8242ef51b5470b1c82d733 |
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Pairwise genomic regions intersection
Pairwise genomic regions intersection ============================================= Overlaps peaks from two ChIP/ATAC experiments |
Path: workflows/peak-intersect.cwl Branch/Commit ID: 261c0232a7a40880f2480b811ed2d7e89c463869 |
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DEPRECATED - Motif Finding with HOMER with target and background regions from peaks
Motif Finding with HOMER with target and background regions from peaks --------------------------------------------------- HOMER contains a novel motif discovery algorithm that was designed for regulatory element analysis in genomics applications (DNA only, no protein). It is a differential motif discovery algorithm, which means that it takes two sets of sequences and tries to identify the regulatory elements that are specifically enriched in on set relative to the other. It uses ZOOPS scoring (zero or one occurrence per sequence) coupled with the hypergeometric enrichment calculations (or binomial) to determine motif enrichment. HOMER also tries its best to account for sequenced bias in the dataset. It was designed with ChIP-Seq and promoter analysis in mind, but can be applied to pretty much any nucleic acids motif finding problem. For more information please refer to: ------------------------------------- [Official documentation](http://homer.ucsd.edu/homer/motif/) |
Path: workflows/homer-motif-analysis-peak.cwl Branch/Commit ID: 261c0232a7a40880f2480b811ed2d7e89c463869 |
