Explore Workflows
View already parsed workflows here or click here to add your own
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any-type-compat.cwl
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Path: tests/any-type-compat.cwl Branch/Commit ID: 707ebcd2173889604459c5f4ffb55173c508abb3 |
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analysis for assembled sequences
rna / protein - qc, annotation, index, abundance |
Path: CWL/Workflows/assembled.workflow.cwl Branch/Commit ID: 963ca6a73a9f8879d57c08fa59548f98f28e755a |
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Whole genome alignment and somatic variant detection
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Path: definitions/pipelines/somatic_wgs.cwl Branch/Commit ID: 8438316338e66823e1c9aca9f675b2bf33f2aa59 |
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bismark-methylation-se.cwl
Bismark Methylation pipeline. We can use indices_folder as genome_folder for bismark_extract_methylation step, because it insludes the original FASTA files too. |
Path: workflows/bismark-methylation-se.cwl Branch/Commit ID: bc75349ad3a7bdce82b4cd8584501f4d0280bb8d |
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RNA-Seq pipeline single-read
The original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for a **single-read** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the single-read RNA-Seq data. It performs the following steps: 1. Use STAR to align reads from input FASTQ file according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 2. Use fastx_quality_stats to analyze input FASTQ file and generate quality statistics file 3. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ file to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
Path: workflows/rnaseq-se.cwl Branch/Commit ID: 42dc4f70b117e78785b82865ec4c4b941ac1c259 |
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bam to trimmed fastqs and biscuit alignments
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Path: definitions/subworkflows/bam_to_trimmed_fastq_and_biscuit_alignments.cwl Branch/Commit ID: 4a04ad33e311c5e647cef848b74034477cb3c47e |
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scatter-valuefrom-wf1.cwl
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Path: cwltool/schemas/v1.0/v1.0/scatter-valuefrom-wf1.cwl Branch/Commit ID: 4635090ef98247b1902b3c7a25c007d9db1cb883 |
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Generate genome indices for STAR & bowtie
Creates indices for: * [STAR](https://github.com/alexdobin/STAR) v2.5.3a (03/17/2017) PMID: [23104886](https://www.ncbi.nlm.nih.gov/pubmed/23104886) * [bowtie](http://bowtie-bio.sourceforge.net/tutorial.shtml) v1.2.0 (12/30/2016) It performs the following steps: 1. `STAR --runMode genomeGenerate` to generate indices, based on [FASTA](http://zhanglab.ccmb.med.umich.edu/FASTA/) and [GTF](http://mblab.wustl.edu/GTF2.html) input files, returns results as an array of files 2. Outputs indices as [Direcotry](http://www.commonwl.org/v1.0/CommandLineTool.html#Directory) data type 3. Separates *chrNameLength.txt* file from Directory output 4. `bowtie-build` to generate indices requires genome [FASTA](http://zhanglab.ccmb.med.umich.edu/FASTA/) file as input, returns results as a group of main and secondary files |
Path: workflows/genome-indices.cwl Branch/Commit ID: a0b22644ca178b640fb74849d23b7c631022f0b5 |
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wffail.cwl
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Path: tests/wf/wffail.cwl Branch/Commit ID: 3ed10d0ea7ac57550433a89a92bdbe756bdb0e40 |
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RNA-Seq pipeline single-read
The original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for a **single-read** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the single-read RNA-Seq data. It performs the following steps: 1. Use STAR to align reads from input FASTQ file according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 2. Use fastx_quality_stats to analyze input FASTQ file and generate quality statistics file 3. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ file to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
Path: workflows/rnaseq-se.cwl Branch/Commit ID: 8bf36bfad5624fbc8fc315e82783a44e9e5e4470 |
