Explore Workflows
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Trim Galore RNA-Seq pipeline single-read strand specific
Note: should be updated The original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for a **single-end** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the single-end RNA-Seq data. It performs the following steps: 1. Trim adapters from input FASTQ file 2. Use STAR to align reads from input FASTQ file according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 3. Use fastx_quality_stats to analyze input FASTQ file and generate quality statistics file 4. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ file to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
Path: workflows/trim-rnaseq-se-dutp.cwl Branch/Commit ID: a0b22644ca178b640fb74849d23b7c631022f0b5 |
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Merge, annotate, and generate a TSV for SVs
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Path: definitions/subworkflows/merge_svs.cwl Branch/Commit ID: 0d2f354af9192a56af258a7d2426c7c160f4ec1a |
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Unaligned BAM to BQSR and VCF
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Path: definitions/subworkflows/bam_to_bqsr_no_dup_marking.cwl Branch/Commit ID: 4a04ad33e311c5e647cef848b74034477cb3c47e |
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downsample unaligned BAM and align
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Path: definitions/subworkflows/downsampled_alignment.cwl Branch/Commit ID: 4a04ad33e311c5e647cef848b74034477cb3c47e |
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Cut-n-Run pipeline paired-end
Experimental pipeline for Cut-n-Run analysis. Uses mapping results from the following experiment types: - `chipseq-pe.cwl` - `trim-chipseq-pe.cwl` - `trim-atacseq-pe.cwl` Note, the upstream analyses should not have duplicates removed |
Path: workflows/trim-chipseq-pe-cut-n-run.cwl Branch/Commit ID: 261c0232a7a40880f2480b811ed2d7e89c463869 |
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trim-rnaseq-pe-dutp.cwl
Runs RNA-Seq BioWardrobe basic analysis with strand specific pair-end data file. |
Path: workflows/trim-rnaseq-pe-dutp.cwl Branch/Commit ID: db6abb2735f87614b870fca9990d36ea786cbbce |
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scatter-wf4.cwl#main
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Path: tests/wf/scatter-wf4.cwl Branch/Commit ID: e9c83739a93fa0b18f8dea2f98b632a9e32725c9 Packed ID: main |
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Cell Ranger Build Reference Indices
Cell Ranger Build Reference Indices =================================== |
Path: workflows/cellranger-mkref.cwl Branch/Commit ID: 2caa50434966ebdf4b33e5ca689c2e4df32f9058 |
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scatter-valuefrom-wf5.cwl
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Path: cwltool/schemas/v1.0/v1.0/scatter-valuefrom-wf5.cwl Branch/Commit ID: bfe56f3138e9e6fc0b9b8c06447553d4cea03d59 |
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exome alignment and germline variant detection
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Path: definitions/pipelines/germline_exome_gvcf.cwl Branch/Commit ID: b9e7392e72506cadd898a6ac4db330baf6535ab6 |
