Explore Workflows
View already parsed workflows here or click here to add your own
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etl.cwl
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![]() Path: workflows/bamfastq_align/etl.cwl Branch/Commit ID: 1326fb7fedca91a274fb7596c9052a4d279eacf9 |
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Build Bismark indices
Copy fasta_file file to the folder and run run bismark_genome_preparation script to prepare indices for Bismark Methylation Analysis. Bowtie2 aligner is used by default. The name of the output indices folder is equal to the genome input. |
![]() Path: workflows/bismark-index.cwl Branch/Commit ID: 282762f8bbaea57dd488115745ef798e128bade1 |
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kmer_top_n_extract
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![]() Path: task_types/tt_kmer_top_n_extract.cwl Branch/Commit ID: 609aead9804a8f31fa9b3dbc7e52105aec487f31 |
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scatterfail.cwl
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![]() Path: tests/wf/scatterfail.cwl Branch/Commit ID: f207d168f4e7eb4dd2279840d4062ba75d9c79c3 |
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Trim Galore RNA-Seq pipeline paired-end
The original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for a **pair-end** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the single-end RNA-Seq data. It performs the following steps: 1. Trim adapters from input FASTQ files 2. Use STAR to align reads from input FASTQ files according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 3. Use fastx_quality_stats to analyze input FASTQ files and generate quality statistics files 4. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ files to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
![]() Path: workflows/trim-rnaseq-pe.cwl Branch/Commit ID: a8eaf61c809d76f55780b14f2febeb363cf6373f |
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CLIP-Seq pipeline for single-read experiment NNNNG
CLIP-Seq workflow for single-read experiment. |
![]() Path: workflows/clipseq-se.cwl Branch/Commit ID: e284e3f6dff25037b209895c52f2abd37a1ce1bf |
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allele-vcf-rnaseq-se.cwl
Allele specific RNA-Seq (using vcf) single-read workflow |
![]() Path: workflows/allele-vcf-rnaseq-se.cwl Branch/Commit ID: e284e3f6dff25037b209895c52f2abd37a1ce1bf |
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search.cwl#main
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![]() Path: cwltool/schemas/v1.0/v1.0/search.cwl Branch/Commit ID: e835bc0487fe42fb330b6222c9be65d18dd81ec9 Packed ID: main |
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pcr-bottleneck-coef.cwl
ChIP-seq - map - PCR Bottleneck Coefficients |
![]() Path: v1.0/map/pcr-bottleneck-coef.cwl Branch/Commit ID: 33385c6a820a9d4d18cff6fc3a533ec8e3c11c6e |
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GSEApy - Gene Set Enrichment Analysis in Python
GSEAPY: Gene Set Enrichment Analysis in Python ============================================== Gene Set Enrichment Analysis is a computational method that determines whether an a priori defined set of genes shows statistically significant, concordant differences between two biological states (e.g. phenotypes). GSEA requires as input an expression dataset, which contains expression profiles for multiple samples. While the software supports multiple input file formats for these datasets, the tab-delimited GCT format is the most common. The first column of the GCT file contains feature identifiers (gene ids or symbols in the case of data derived from RNA-Seq experiments). The second column contains a description of the feature; this column is ignored by GSEA and may be filled with “NA”s. Subsequent columns contain the expression values for each feature, with one sample's expression value per column. It is important to note that there are no hard and fast rules regarding how a GCT file's expression values are derived. The important point is that they are comparable to one another across features within a sample and comparable to one another across samples. Tools such as DESeq2 can be made to produce properly normalized data (normalized counts) which are compatible with GSEA. |
![]() Path: workflows/gseapy.cwl Branch/Commit ID: a8eaf61c809d76f55780b14f2febeb363cf6373f |