Explore Workflows
View already parsed workflows here or click here to add your own
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readme-assembly-workflow.cwl
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![]() Path: flow_create_readme/readme-assembly-workflow.cwl Branch/Commit ID: 5910b4d88aca172252d9102ddb610a7dc9e1347f |
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workflow.cwl
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![]() Path: flow_download/workflow.cwl Branch/Commit ID: 5910b4d88aca172252d9102ddb610a7dc9e1347f |
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readme-genePrediction-workflow.cwl
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![]() Path: flow_create_readme/readme-genePrediction-workflow.cwl Branch/Commit ID: 5910b4d88aca172252d9102ddb610a7dc9e1347f |
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workflow.cwl
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![]() Path: flow_dispatch/workflow.cwl Branch/Commit ID: 5910b4d88aca172252d9102ddb610a7dc9e1347f |
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workflow.cwl
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![]() Path: flow_dispatch/2blat/workflow.cwl Branch/Commit ID: 5910b4d88aca172252d9102ddb610a7dc9e1347f |
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SoupX - an R package for the estimation and removal of cell free mRNA contamination
Devel version of Single-Cell Advanced Cell Ranger Pipeline (SoupX) ================================================================= |
![]() Path: workflows/soupx.cwl Branch/Commit ID: 954bb2f213d97dfef1cddaf9e830169a92ad0c6b |
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Trim Galore RNA-Seq pipeline paired-end
The original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for a **pair-end** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the single-end RNA-Seq data. It performs the following steps: 1. Trim adapters from input FASTQ files 2. Use STAR to align reads from input FASTQ files according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 3. Use fastx_quality_stats to analyze input FASTQ files and generate quality statistics files 4. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ files to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
![]() Path: workflows/trim-rnaseq-pe.cwl Branch/Commit ID: 954bb2f213d97dfef1cddaf9e830169a92ad0c6b |
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Compute average of average for core domain instances
Compute average structure for all averaged structures corresponding to core UniProt domain instances. First computes average per UniProt domain instance and then average all averaged structures. |
![]() Path: Tools/core_avg_subwf.cwl Branch/Commit ID: b5a9d4b025ec8e065bae97eeb96f10db2dd8e1e6 |
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RNA-Seq pipeline single-read strand specific
Note: should be updated The original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for **strand specific single-read** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the single-read RNA-Seq data. It performs the following steps: 1. Use STAR to align reads from input FASTQ file according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 2. Use fastx_quality_stats to analyze input FASTQ file and generate quality statistics file 3. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ file to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
![]() Path: workflows/rnaseq-se-dutp.cwl Branch/Commit ID: 954bb2f213d97dfef1cddaf9e830169a92ad0c6b |
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Single-cell Multiome ATAC and RNA-Seq Alignment
Single-cell Multiome ATAC and RNA-Seq Alignment Runs Cell Ranger ARC Count to quantifies chromatin accessibility and gene expression from a single-cell Multiome ATAC and RNA-Seq library |
![]() Path: workflows/sc-multiome-align-wf.cwl Branch/Commit ID: 8614e5d20f5e81dce537216bd340cdbc1067bbc7 |