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Graph Name Retrieved From View
workflow graph Interval overlapping alignments counts

Interval overlapping alignments counts ====================================== Reports the count of alignments from multiple samples that overlap specific intervals.

 https://github.com/datirium/workflows.git

Path: workflows/bedtools-multicov.cwl

Branch/Commit ID: 93b844a80f4008cc973ea9b5efedaff32a343895
workflow graph miRNA-Seq miRDeep2 pipeline

A CWL workflow for discovering known or novel miRNAs from deep sequencing data using the miRDeep2 tool. The ExoCarta exosome database is also used for identifying exosome-related miRNAs, and TargetScan's organism-specific databases are used for identifying miRNA gene targets. ## __Outputs__ #### Primary Output files: - mirs_known.tsv, detected known mature miRNAs, \"Known miRNAs\" tab - mirs_novel.tsv, detected novel mature miRNAs, \"Novel miRNAs\" tab #### Secondary Output files: - mirs_known_exocarta_deepmirs.tsv, list of detected miRNA also in ExoCarta's exosome database, \"Detected Exosome miRNAs\" tab - mirs_known_gene_targets.tsv, pre-computed gene targets of known mature mirs, downloadable - known_mirs_mature.fa, known mature mir sequences, downloadable - known_mirs_precursor.fa, known precursor mir sequences, downloadable - novel_mirs_mature.fa, novel mature mir sequences, downloadable - novel_mirs_precursor.fa, novel precursor mir sequences, downloadable #### Reports: - overview.md (input list, alignment & mir metrics), \"Overview\" tab - mirdeep2_result.html, summary of mirdeep2 results, \"miRDeep2 Results\" tab ## __Inputs__ #### General Info - Sample short name/Alias: unique name for sample - Experimental condition: condition, variable, etc name (e.g. \"control\" or \"20C 60min\") - Cells: name of cells used for the sample - Catalog No.: vender catalog number if available - Bowtie2 index: Bowtie2 index directory of the reference genome. - Reference Genome FASTA: Reference genome FASTA file to be used for alignment. - Genome short name: Name used for setting organism name, genus, species, and tax ID. - Input FASTQ file: FASTQ file from a single-end miRNA sequencing run. #### Advanced - Adapter: Adapter sequence to be trimmed from miRNA sequence reads. (Default: TCGTAT) - Threads: Number of threads to use for steps that support multithreading (Default: 4). ## Hints & Tips: #### For the identification of novel miRNA candidates, the following may be used as a filtering guideline: 1. miRDeep score > 4 (some authors use 1) 2. not present a match with rfam 3. should present a significant RNAfold (\"yes\") 4. a number of mature reads > 10 5. if applicable, novel mir must be expressed in multiple samples #### For filtering mirbase by organism. | genome | organism | division | name | tree | NCBI-taxid | | ---- | --- | --- | ----------- | ----------- | ----------- | | hg19 | hsa | HSA | Homo sapiens | Metazoa;Bilateria;Deuterostoma;Chordata;Vertebrata;Mammalia;Primates;Hominidae | 9606 | | hg38 | hsa | HSA | Homo sapiens | Metazoa;Bilateria;Deuterostoma;Chordata;Vertebrata;Mammalia;Primates;Hominidae | 9606 | | mm10 | mmu | MMU | Mus musculus | Metazoa;Bilateria;Deuterostoma;Chordata;Vertebrata;Mammalia;Rodentia | 10090 | | rn7 | rno | RNO | Rattus norvegicus | Metazoa;Bilateria;Deuterostoma;Chordata;Vertebrata;Mammalia;Rodentia | 10116 | | dm3 | dme | DME | Drosophila melanogaster | Metazoa;Bilateria;Ecdysozoa;Arthropoda;Hexapoda | 7227 | ## __Data Analysis Steps__ 1. The miRDeep2 Mapper module processes Illumina FASTQ output and maps it to the reference genome. 2. The miRDeep2 miRDeep2 module identifies known and novel (mature and precursor) miRNAs. 3. The ExoCarta database of miRNA found in exosomes is then used to find overlap between mirs_known.tsv and exosome associated miRNAs. 4. Finally, TargetScan organism-specific miRNA gene target database is used to find overlap between mirs_known.tsv and gene targets. ## __References__ 1. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3245920 2. https://github.com/rajewsky-lab/mirdeep2 3. https://biocontainers.pro/tools/mirdeep2 4. https://www.mirbase.org/ 5. http://exocarta.org/index.html 6. https://www.targetscan.org/vert_80/

https://github.com/datirium/workflows.git

Path: workflows/mirna-mirdeep2-se.cwl

Branch/Commit ID: 93b844a80f4008cc973ea9b5efedaff32a343895
workflow graph DESeq2 Multi-factor Analysis

DESeq2 Multi-factor Analysis Runs DeSeq2 multi-factor analysis with manual control over major parameters

 https://github.com/datirium/workflows.git

Path: workflows/deseq-multi-factor.cwl

Branch/Commit ID: 93b844a80f4008cc973ea9b5efedaff32a343895
workflow graph BWA index pipeline

This workflow indexes the input reference FASTA with bwa, and generates faidx and dict file using samtools. This index sample can then be used as input into the germline variant calling workflow, or others that may include this workflow as an upstream source. ### __Inputs__ - FASTA file of the reference genome that will be indexed. ### __Outputs__ - Directory containing the original FASTA, faidx, dict, and bwa index files. - stdout log file (output in Overview tab as well) - stderr log file ### __Data Analysis Steps__ 1. cwl calls dockercontainer robertplayer/scidap-gatk4 to index reference FASTA with bwa, and generates faidx and dict files using samtools ### __References__ - Li, H., & Durbin, R. (2009). Fast and accurate short read alignment with Burrows–Wheeler transform. Bioinformatics, 25(14), 1754–1760.

https://github.com/datirium/workflows.git

Path: workflows/bwa-index.cwl

Branch/Commit ID: 93b844a80f4008cc973ea9b5efedaff32a343895
workflow graph taxonomy_check_16S

https://github.com/ncbi/pgap.git

Path: task_types/tt_taxonomy_check_16S.cwl

Branch/Commit ID: a402541b8530f30eab726c160da90a23036847a1
workflow graph js-expr-req-wf.cwl#wf

https://github.com/common-workflow-language/cwltool.git

Path: cwltool/schemas/v1.0/v1.0/js-expr-req-wf.cwl

Branch/Commit ID: 09323506da219ba3ddb5313bd83022b52cac9adc

Packed ID: wf
workflow graph Workflow to run pVACseq from detect_variants and rnaseq pipeline outputs

https://github.com/genome/analysis-workflows.git

Path: definitions/subworkflows/pvacseq.cwl

Branch/Commit ID: 4bc0a4577d626b65a4b44683e5a1ab2f7d7faf4c
workflow graph Filter differentially expressed genes from DESeq for Tag Density Profile Analyses

Filters differentially expressed genes from DESeq for Tag Density Profile Analyses ================================================================================== Tool filters output from DESeq pipeline run for genes to create a file with regions of interest for Tag Density Profile Analyses.

https://github.com/datirium/workflows.git

Path: workflows/filter-deseq-for-heatmap.cwl

Branch/Commit ID: 93b844a80f4008cc973ea9b5efedaff32a343895
workflow graph rnaseq-se-dutp.cwl

Runs RNA-Seq dUTP BioWardrobe basic analysis with strand specific single-end data file.

https://github.com/Barski-lab/workflows.git

Path: workflows/rnaseq-se-dutp.cwl

Branch/Commit ID: bc75349ad3a7bdce82b4cd8584501f4d0280bb8d
workflow graph preprocess fasta

Remove reads from fasta files based on sequence stats. Return fasta files with reads passed and reads removed.

https://github.com/MG-RAST/pipeline.git

Path: CWL/Workflows/preprocess-fasta.workflow.cwl

Branch/Commit ID: f906212e2c9a88280ae36545e5422f25752aa8f4