Explore Workflows
View already parsed workflows here or click here to add your own
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umi molecular alignment workflow
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Path: definitions/subworkflows/molecular_qc.cwl Branch/Commit ID: 31602b94b34ff55876147c7299e1bec47e8d1a31 |
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rnaseq-se-dutp.cwl
Runs RNA-Seq dUTP BioWardrobe basic analysis with strand specific single-end data file. |
Path: workflows/rnaseq-se-dutp.cwl Branch/Commit ID: 812b0ff40dda18ab7a9a872ff13a577be8531ba6 |
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Motif Finding with HOMER with custom background regions
Motif Finding with HOMER with custom background regions --------------------------------------------------- HOMER contains a novel motif discovery algorithm that was designed for regulatory element analysis in genomics applications (DNA only, no protein). It is a differential motif discovery algorithm, which means that it takes two sets of sequences and tries to identify the regulatory elements that are specifically enriched in on set relative to the other. It uses ZOOPS scoring (zero or one occurrence per sequence) coupled with the hypergeometric enrichment calculations (or binomial) to determine motif enrichment. HOMER also tries its best to account for sequenced bias in the dataset. It was designed with ChIP-Seq and promoter analysis in mind, but can be applied to pretty much any nucleic acids motif finding problem. For more information please refer to: ------------------------------------- [Official documentation](http://homer.ucsd.edu/homer/motif/) |
Path: workflows/homer-motif-analysis-bg.cwl Branch/Commit ID: 36fd18f11e939d3908b1eca8d2939402f7a99b0f |
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MAnorm PE - quantitative comparison of ChIP-Seq paired-end data
What is MAnorm? -------------- MAnorm is a robust model for quantitative comparison of ChIP-Seq data sets of TFs (transcription factors) or epigenetic modifications and you can use it for: * Normalization of two ChIP-seq samples * Quantitative comparison (differential analysis) of two ChIP-seq samples * Evaluating the overlap enrichment of the protein binding sites(peaks) * Elucidating underlying mechanisms of cell-type specific gene regulation How MAnorm works? ---------------- MAnorm uses common peaks of two samples as a reference to build the rescaling model for normalization, which is based on the empirical assumption that if a chromatin-associated protein has a substantial number of peaks shared in two conditions, the binding at these common regions will tend to be determined by similar mechanisms, and thus should exhibit similar global binding intensities across samples. The observed differences on common peaks are presumed to reflect the scaling relationship of ChIP-Seq signals between two samples, which can be applied to all peaks. What do the inputs mean? ---------------- ### General **Experiment short name/Alias** * short name for you experiment to identify among the others **ChIP-Seq PE sample 1** * previously analyzed ChIP-Seq paired-end experiment to be used as Sample 1 **ChIP-Seq PE sample 2** * previously analyzed ChIP-Seq paired-end experiment to be used as Sample 2 **Genome** * Reference genome to be used for gene assigning ### Advanced **Reads shift size for sample 1** * This value is used to shift reads towards 3' direction to determine the precise binding site. Set as half of the fragment length. Default 100 **Reads shift size for sample 2** * This value is used to shift reads towards 5' direction to determine the precise binding site. Set as half of the fragment length. Default 100 **M-value (log2-ratio) cutoff** * Absolute M-value (log2-ratio) cutoff to define biased (differential binding) peaks. Default: 1.0 **P-value cutoff** * P-value cutoff to define biased peaks. Default: 0.01 **Window size** * Window size to count reads and calculate read densities. 2000 is recommended for sharp histone marks like H3K4me3 and H3K27ac, and 1000 for TFs or DNase-seq. Default: 2000 |
Path: workflows/manorm-pe.cwl Branch/Commit ID: f3e44d3b0f198cf5245c49011124dc3b6c2b06fd |
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bam_readcount workflow
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Path: definitions/subworkflows/bam_readcount.cwl Branch/Commit ID: 44ada20f3eeb59005d5bd999d2435102e9bae991 |
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tt_kmer_compare_wnode
Pairwise comparison |
Path: task_types/tt_kmer_compare_wnode.cwl Branch/Commit ID: 1c1f5aa0b0dfe5b07beb8ccb0c0e3c073a9fb592 |
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exome alignment and tumor-only variant detection
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Path: definitions/pipelines/tumor_only_exome.cwl Branch/Commit ID: 788bdc99c1d5b6ee7c431c3c011eb30d385c1370 |
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three_step_color.cwl
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Path: tests/wf/three_step_color.cwl Branch/Commit ID: 6c86caa0571fd186d90a6600e0bb405596d4a5e0 |
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downsample unaligned BAM and align
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Path: definitions/subworkflows/downsampled_alignment.cwl Branch/Commit ID: 31602b94b34ff55876147c7299e1bec47e8d1a31 |
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varscan somatic workflow
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Path: definitions/subworkflows/varscan.cwl Branch/Commit ID: 60edaf6f57eaaf02cda1a3d8cb9a825aa64a43e2 |
