Explore Workflows
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Single-Cell Label Integration Analysis
Single-Cell Label Integration Analysis Harmonizes conflicting annotations in single-cell genomics studies. |
Path: workflows/sc-triangulate.cwl Branch/Commit ID: 93b844a80f4008cc973ea9b5efedaff32a343895 |
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Single-Cell ATAC-Seq Dimensionality Reduction Analysis
Single-Cell ATAC-Seq Dimensionality Reduction Analysis Removes noise and confounding sources of variation by reducing dimensionality of chromatin accessibility data from the outputs of “Single-Cell Multiome ATAC and RNA-Seq Filtering Analysis” pipelines. The results of this workflow are primarily used in “Single-Cell ATAC-Seq Cluster Analysis” or “Single-Cell WNN Cluster Analysis” pipelines. |
Path: workflows/sc-atac-reduce.cwl Branch/Commit ID: 93b844a80f4008cc973ea9b5efedaff32a343895 |
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Seed Protein Alignments
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Path: protein_alignment/wf_seed_seqids.cwl Branch/Commit ID: 424a01693259a75641dc249d553235aa38a6ce23 |
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Single-Cell RNA-Seq Cluster Analysis
Single-Cell RNA-Seq Cluster Analysis Clusters cells by similarity of gene expression data from the outputs of the “Single-Cell RNA-Seq Dimensionality Reduction Analysis” pipeline. The results of this workflow are used in the “Single-Cell Manual Cell Type Assignment”, “Single-Cell RNA-Seq Differential Expression Analysis”, “Single-Cell RNA-Seq Trajectory Analysis”, and “Single-Cell Differential Abundance Analysis” pipelines. |
Path: workflows/sc-rna-cluster.cwl Branch/Commit ID: 93b844a80f4008cc973ea9b5efedaff32a343895 |
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Kallisto index pipeline
This workflow indexes the input reference FASTA with kallisto, and generates a kallisto index file (.kdx). This index sample can then be used as input into the kallisto transcript-level quantification workflow (kallisto-quant-pe.cwl), or others that may include this workflow as an upstream source. ### __Inputs__ - FASTA file of the reference genome that will be indexed - number of threads to use for multithreading processes ### __Outputs__ - kallisto index file (.kdx). - stdout log file (output in Overview tab as well) - stderr log file ### __Data Analysis Steps__ 1. cwl calls dockercontainer robertplayer/scidap-kallisto to index reference FASTA with `kallisto index`, generating a kallisto index file. ### __References__ - Bray, N. L., Pimentel, H., Melsted, P. & Pachter, L. Near-optimal probabilistic RNA-seq quantification, Nature Biotechnology 34, 525-527(2016), doi:10.1038/nbt.3519 |
Path: workflows/kallisto-index.cwl Branch/Commit ID: 93b844a80f4008cc973ea9b5efedaff32a343895 |
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Cell Ranger Aggregate (RNA, RNA+VDJ)
Cell Ranger Aggregate (RNA, RNA+VDJ) Combines outputs from multiple runs of either “Cell Ranger Count (RNA)” or “Cell Ranger Count (RNA+VDJ)” pipelines. The results of this workflow are primarily used in “Single-Cell RNA-Seq Filtering Analysis” and “Single-Cell Immune Profiling Analysis” pipelines. |
Path: workflows/cellranger-aggr.cwl Branch/Commit ID: 93b844a80f4008cc973ea9b5efedaff32a343895 |
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Align reference proteins plane complete workflow
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Path: protein_alignment/wf_protein_alignment.cwl Branch/Commit ID: 424a01693259a75641dc249d553235aa38a6ce23 |
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Cell Ranger Count (RNA+VDJ)
Cell Ranger Count (RNA+VDJ) Quantifies single-cell gene expression, performs V(D)J contigs assembly and clonotype calling of the sequencing data from a single 10x Genomics library in a combined manner. The results of this workflow are primarily used in either “Single-Cell RNA-Seq Filtering Analysis”, “Single-Cell Immune Profiling Analysis”, or “Cell Ranger Aggregate (RNA, RNA+VDJ)” pipelines. |
Path: workflows/cellranger-multi.cwl Branch/Commit ID: 93b844a80f4008cc973ea9b5efedaff32a343895 |
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Cell Ranger Count (RNA)
Cell Ranger Count (RNA) Quantifies single-cell gene expression of the sequencing data from a single 10x Genomics library. The results of this workflow are primarily used in either “Single-Cell RNA-Seq Filtering Analysis” or “Cell Ranger Aggregate (RNA, RNA+VDJ)” pipelines. |
Path: workflows/single-cell-preprocess-cellranger.cwl Branch/Commit ID: 93b844a80f4008cc973ea9b5efedaff32a343895 |
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super-enhancer.cwl
Both `islands_file` and `islands_control_file` should be produced by the same cwl tool (iaintersect.cwl or macs2-callpeak-biowardrobe-only.cwl) |
Path: workflows/super-enhancer.cwl Branch/Commit ID: 801f7b363e0599b9a28ecda696dfdb1c0e40ce71 |
