Explore Workflows
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output-arrays-int-wf.cwl
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![]() Path: tests/output-arrays-int-wf.cwl Branch/Commit ID: 707ebcd2173889604459c5f4ffb55173c508abb3 |
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exome alignment and tumor-only variant detection
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![]() Path: definitions/pipelines/tumor_only_exome.cwl Branch/Commit ID: 8da2b1cd6fa379b2c22baf9dad762d39630e6f46 |
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align_merge_sas
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![]() Path: task_types/tt_align_merge_sas.cwl Branch/Commit ID: 093b60e546237c06cfe7820d6ac8d66467e66725 |
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mut.cwl
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![]() Path: tests/wf/mut.cwl Branch/Commit ID: 10492acee927c177933160f6ad67085f9112b0d1 |
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RNA-Seq pipeline paired-end stranded mitochondrial
Slightly changed original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for **strand specific pair-end** experiment. An additional steps were added to map data to mitochondrial chromosome only and then merge the output. Experiment files in [FASTQ](http://maq.sourceforge.net/fastq.shtml) format either compressed or not can be used. Current workflow should be used only with the pair-end strand specific RNA-Seq data. It performs the following steps: 1. `STAR` to align reads from input FASTQ file according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 2. `fastx_quality_stats` to analyze input FASTQ file and generate quality statistics file 3. `samtools sort` to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ file to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using `GEEP` reads-counting utility; export results to file |
![]() Path: workflows/rnaseq-pe-dutp-mitochondrial.cwl Branch/Commit ID: dda9e6e06a656b7b3fa7504156474b962fe3953c |
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blastp_wnode_naming
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![]() Path: task_types/tt_blastp_wnode_naming.cwl Branch/Commit ID: 1b8d71c75156a1a62bf0477d59db26010e2dcc29 |
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extract_gencoll_ids
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![]() Path: task_types/tt_extract_gencoll_ids.cwl Branch/Commit ID: 550682d2fe3348161eab1b8612e48a59af4ac6a5 |
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mut.cwl
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![]() Path: tests/wf/mut.cwl Branch/Commit ID: b82ce7ae901a54c7a062fd5eefd8d5ceb5a4d684 |
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phase VCF
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![]() Path: definitions/subworkflows/phase_vcf.cwl Branch/Commit ID: d2c2f2eb846ae2e9cdcab46e3bb88e42126cb3f5 |
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facets-workflow.cwl
Workflow for running Facets-suite on a set of tumor normal pairs This workflow scatters over all the pairs in the input JSON to run all samples in parallel Input JSON format ----------------- { \"pairs\": [ { \"tumor_bam\": { \"class\": \"File\", \"path\": \"/test_data/bam/Tumor1.rg.md.abra.printreads.bam\" }, \"normal_bam\": { \"class\": \"File\", \"path\": \"/test_data/bam/Normal1.rg.md.abra.printreads.bam\" }, \"pair_maf\": { \"class\": \"File\", \"path\": \"/test_data/bam/Tumor1.Normal1.maf\" }, \"pair_id\": \"Tumor1.Normal1\" }, { \"tumor_bam\": { \"class\": \"File\", \"path\": \"/test_data/bam/Tumor2.rg.md.abra.printreads.bam\" }, \"normal_bam\": { \"class\": \"File\", \"path\": \"/test_data/bam/Normal2.rg.md.abra.printreads.bam\" }, \"pair_maf\": { \"class\": \"File\", \"path\": \"/test_data/bam/Tumor2.Normal2.maf\" }, \"pair_id\": \"Tumor2.Normal2\" } ] } Output format ------------- output └── facets-suite ├── Tumor1.Normal1.arm_level.txt ├── Tumor1.Normal1.gene_level.txt ├── Tumor1.Normal1_hisens.ccf.maf ├── Tumor1.Normal1_hisens.rds ├── Tumor1.Normal1_hisens.seg ├── Tumor1.Normal1_purity.rds ├── Tumor1.Normal1_purity.seg ├── Tumor1.Normal1.qc.txt ├── Tumor1.Normal1.snp_pileup.gz ├── Tumor1.Normal1.txt ├── Tumor2.Normal2.arm_level.txt ├── Tumor2.Normal2.gene_level.txt ├── Tumor2.Normal2_hisens.ccf.maf ├── Tumor2.Normal2_hisens.rds ├── Tumor2.Normal2_hisens.seg ├── Tumor2.Normal2_purity.rds ├── Tumor2.Normal2_purity.seg ├── Tumor2.Normal2.qc.txt ├── Tumor2.Normal2.snp_pileup.gz ├── Tumor2.Normal2.txt └── logs ├── success └── failed |
![]() Path: cwl/facets-workflow.cwl Branch/Commit ID: 462f6015c9268a4205b6e81de018a470b8a4a153 |