Explore Workflows
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DiffBind - Differential Binding Analysis of ChIP-Seq or CUTß&RUN/Tag Peak Data
Differential Binding Analysis of ChIP-Seq or CUT&RUN/Tag Peak Data --------------------------------------------------- DiffBind processes ChIP-Seq or CUT&RUN/Tag data enriched for genomic loci where specific protein/DNA binding occurs, including peak sets identified by peak caller tools and aligned sequence read datasets. It is designed to work with multiple peak sets simultaneously, representing different ChIP or CUT&RUN/Tag experiments (antibodies, transcription factor and/or histone marks, experimental conditions, replicates) as well as managing the results of multiple peak callers. For more information please refer to: ------------------------------------- Ross-Innes CS, Stark R, Teschendorff AE, Holmes KA, Ali HR, Dunning MJ, Brown GD, Gojis O, Ellis IO, Green AR, Ali S, Chin S, Palmieri C, Caldas C, Carroll JS (2012). “Differential oestrogen receptor binding is associated with clinical outcome in breast cancer.” Nature, 481, -4. |
![]() Path: workflows/diffbind.cwl Branch/Commit ID: 549fac35bf6b8b1c25af0f4f6c3f162c40dc130e |
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diadem_workflow_test1.cwl
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![]() Path: diadem_workflow_test1.cwl Branch/Commit ID: b3583f47fdbc2a1252b847cdf67470da80c14302 |
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RNA-Seq pipeline paired-end stranded mitochondrial
Slightly changed original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for **strand specific pair-end** experiment. An additional steps were added to map data to mitochondrial chromosome only and then merge the output. Experiment files in [FASTQ](http://maq.sourceforge.net/fastq.shtml) format either compressed or not can be used. Current workflow should be used only with the pair-end strand specific RNA-Seq data. It performs the following steps: 1. `STAR` to align reads from input FASTQ file according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 2. `fastx_quality_stats` to analyze input FASTQ file and generate quality statistics file 3. `samtools sort` to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ file to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using `GEEP` reads-counting utility; export results to file |
![]() Path: workflows/rnaseq-pe-dutp-mitochondrial.cwl Branch/Commit ID: 42dc4f70b117e78785b82865ec4c4b941ac1c259 |
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scatter2.cwl
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![]() Path: tests/wf/scatter2.cwl Branch/Commit ID: 7bfe73a708dbf31d037303bb5a8fed1a79984b0f |
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mutect parallel workflow
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![]() Path: definitions/subworkflows/mutect.cwl Branch/Commit ID: b9e7392e72506cadd898a6ac4db330baf6535ab6 |
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wgs alignment and tumor-only variant detection
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![]() Path: definitions/pipelines/tumor_only_wgs.cwl Branch/Commit ID: 40097e1ed094c5b42b68f3db2ff2cbe78c182479 |
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bam-bedgraph-bigwig.cwl
Workflow converts input BAM file into bigWig and bedGraph files. Input BAM file should be sorted by coordinates (required by `bam_to_bedgraph` step). If `split` input is not provided use true by default. Default logic is implemented in `valueFrom` field of `split` input inside `bam_to_bedgraph` step to avoid possible bug in cwltool with setting default values for workflow inputs. `scale` has higher priority over the `mapped_reads_number`. The last one is used to calculate `-scale` parameter for `bedtools genomecov` (step `bam_to_bedgraph`) only in a case when input `scale` is not provided. All logic is implemented inside `bedtools-genomecov.cwl`. `bigwig_filename` defines the output name only for generated bigWig file. `bedgraph_filename` defines the output name for generated bedGraph file and can influence on generated bigWig filename in case when `bigwig_filename` is not provided. All workflow inputs and outputs don't have `format` field to avoid format incompatibility errors when workflow is used as subworkflow. |
![]() Path: tools/bam-bedgraph-bigwig.cwl Branch/Commit ID: 80d64741638b14de5cf58236b6d6d99713c62086 |
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abundance
abundace profiles from annotated files, for protein and/or rna |
![]() Path: CWL/Workflows/abundance-clca.workflow.cwl Branch/Commit ID: 6a8727124baf77416ca797982fd4e0689c2a593a |
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assm_assm_blastn_wnode
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![]() Path: task_types/tt_assm_assm_blastn_wnode.cwl Branch/Commit ID: 55b6ee46b0c9fb1c9949cd0888b388c6f11b73b1 |
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index sim seq
create sorted / filtered similarity file with feature sequences, and index by md5 |
![]() Path: CWL/Workflows/index_sim_seq.workflow.cwl Branch/Commit ID: 6a8727124baf77416ca797982fd4e0689c2a593a |