Explore Workflows
View already parsed workflows here or click here to add your own
| Graph | Name | Retrieved From | View |
|---|---|---|---|
|
|
mut.cwl
|
Path: tests/wf/mut.cwl Branch/Commit ID: 6b8f06a9f6f6a570142c7aedc767fea2efa2a0cc |
|
|
|
cond-single-source-wf-005.1.cwl
|
Path: testdata/cond-single-source-wf-005.1.cwl Branch/Commit ID: b926e330eba795f3acc1f71fd0645e75f925a2da |
|
|
|
gp_makeblastdb
|
Path: progs/gp_makeblastdb.cwl Branch/Commit ID: 369e2b6c7f4db75099d258729dec1326f55d2cc5 |
|
|
|
Filter ChIP/ATAC peaks for Tag Density Profile or Motif Enrichment analyses
Filters ChIP/ATAC peaks with the neatest genes assigned for Tag Density Profile or Motif Enrichment analyses ============================================================================================================ Tool filters output from any ChIP/ATAC pipeline to create a file with regions of interest for Tag Density Profile or Motif Enrichment analyses. Peaks with duplicated coordinates are discarded. |
Path: workflows/filter-peaks-for-heatmap.cwl Branch/Commit ID: 36fd18f11e939d3908b1eca8d2939402f7a99b0f |
|
|
|
Trim Galore RNA-Seq pipeline paired-end
The original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for a **pair-end** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow must be used with paired-end RNA-Seq data. It performs the following steps: 1. Trim adapters from input FASTQ files 2. Use STAR to align reads from input FASTQ files according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 3. Use fastx_quality_stats to analyze input FASTQ files and generate quality statistics files 4. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 2 (after running STAR) 5. Generate BigWig file using sorted BAM file 6. Map input FASTQ files to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
Path: workflows/trim-rnaseq-pe.cwl Branch/Commit ID: 57863b6131d8262c5ce864adaf8e4038401e71a2 |
|
|
|
DESeq - differential gene expression analysis
Differential gene expression analysis ===================================== Differential gene expression analysis based on the negative binomial distribution Estimate variance-mean dependence in count data from high-throughput sequencing assays and test for differential expression based on a model using the negative binomial distribution. DESeq1 ------ High-throughput sequencing assays such as RNA-Seq, ChIP-Seq or barcode counting provide quantitative readouts in the form of count data. To infer differential signal in such data correctly and with good statistical power, estimation of data variability throughout the dynamic range and a suitable error model are required. Simon Anders and Wolfgang Huber propose a method based on the negative binomial distribution, with variance and mean linked by local regression and present an implementation, [DESeq](http://bioconductor.org/packages/release/bioc/html/DESeq.html), as an R/Bioconductor package DESeq2 ------ In comparative high-throughput sequencing assays, a fundamental task is the analysis of count data, such as read counts per gene in RNA-seq, for evidence of systematic changes across experimental conditions. Small replicate numbers, discreteness, large dynamic range and the presence of outliers require a suitable statistical approach. [DESeq2](http://www.bioconductor.org/packages/release/bioc/html/DESeq2.html), a method for differential analysis of count data, using shrinkage estimation for dispersions and fold changes to improve stability and interpretability of estimates. This enables a more quantitative analysis focused on the strength rather than the mere presence of differential expression. |
Path: workflows/deseq.cwl Branch/Commit ID: f3e44d3b0f198cf5245c49011124dc3b6c2b06fd |
|
|
|
step_valuefrom5_wf_v1_2.cwl
|
Path: testdata/step_valuefrom5_wf_v1_2.cwl Branch/Commit ID: 0ab1d42d10f7311bb4032956c4a6f3d2730d9507 |
|
|
|
Feature expression merge - combines feature expression from several experiments
Feature expression merge - combines feature expression from several experiments ========================================================================= Workflows merges RPKM (by default) gene expression from several experiments based on the values from GeneId, Chrom, TxStart, TxEnd and Strand columns (by default). Reported unique columns are renamed based on the experiments names. |
Path: workflows/feature-merge.cwl Branch/Commit ID: 57863b6131d8262c5ce864adaf8e4038401e71a2 |
|
|
|
wgs alignment with qc
|
Path: definitions/pipelines/alignment_wgs.cwl Branch/Commit ID: adcae308fdccaa1190083616118dfadb4df65dca |
|
|
|
Seed Protein Alignments
|
Path: protein_alignment/wf_seed_seqids.cwl Branch/Commit ID: cb15f907132fb90bc66b39bb0af3c211801feba1 |
