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Graph Name Retrieved From View
workflow graph Detect Variants workflow for WGS pipeline

https://github.com/genome/analysis-workflows.git

Path: definitions/pipelines/detect_variants_wgs.cwl

Branch/Commit ID: 9143dc4ebacb9e1df36a712b0be6fa5d982b0c4f
workflow graph Generate ATDP heatmap using Homer

Generate ATDP heatmap centered on TSS from an array of input BAM files and genelist TSV file. Returns array of heatmap JSON files with the names that have the same basenames as input BAM files, but with .json extension

https://github.com/datirium/workflows.git

Path: workflows/heatmap.cwl

Branch/Commit ID: 9ee330737f4603e4e959ffe786fbb2046db70a00
workflow graph count-lines4-wf.cwl

https://github.com/common-workflow-language/cwltool.git

Path: cwltool/schemas/v1.0/v1.0/count-lines4-wf.cwl

Branch/Commit ID: 2ae8117360a3cd4909d9d3f2b35c30bfffb25d0a
workflow graph Variant calling germline paired-end

A workflow for the Broad Institute's best practices gatk4 germline variant calling pipeline. ## __Outputs__ #### Primary Output files: - bqsr2_indels.vcf, filtered and recalibrated indels (IGV browser) - bqsr2_snps.vcf, filtered and recalibrated snps (IGV browser) - bqsr2_snps.ann.vcf, filtered and recalibrated snps with effect annotations #### Secondary Output files: - sorted_dedup_reads.bam, sorted deduplicated alignments (IGV browser) - raw_indels.vcf, first pass indel calls - raw_snps.vcf, first pass snp calls #### Reports: - overview.md (input list, alignment metrics, variant counts) - insert_size_histogram.pdf - recalibration_plots.pdf - snpEff_summary.html ## __Inputs__ #### General Info - Sample short name/Alias: unique name for sample - Experimental condition: condition, variable, etc name (e.g. \"control\" or \"20C 60min\") - Cells: name of cells used for the sample - Catalog No.: vender catalog number if available - BWA index: BWA index sample that contains reference genome FASTA with associated indices. - SNPEFF database: Name of SNPEFF database to use for SNP effect annotation. - Read 1 file: First FASTQ file (generally contains \"R1\" in the filename) - Read 2 file: Paired FASTQ file (generally contains \"R2\" in the filename) #### Advanced - Ploidy: number of copies per chromosome (default should be 2) - SNP filters: see Step 6 Notes: https://gencore.bio.nyu.edu/variant-calling-pipeline-gatk4/ - Indel filters: see Step 7 Notes: https://gencore.bio.nyu.edu/variant-calling-pipeline-gatk4/ #### SNPEFF notes: Get snpeff databases using `docker run --rm -ti gatk4-dev /bin/bash` then running `java -jar $SNPEFF_JAR databases`. Then, use the first column as SNPEFF input (e.g. \"hg38\"). - hg38, Homo_sapiens (USCS), http://downloads.sourceforge.net/project/snpeff/databases/v4_3/snpEff_v4_3_hg38.zip - mm10, Mus_musculus, http://downloads.sourceforge.net/project/snpeff/databases/v4_3/snpEff_v4_3_mm10.zip - dm6.03, Drosophila_melanogaster, http://downloads.sourceforge.net/project/snpeff/databases/v4_3/snpEff_v4_3_dm6.03.zip - Rnor_6.0.86, Rattus_norvegicus, http://downloads.sourceforge.net/project/snpeff/databases/v4_3/snpEff_v4_3_Rnor_6.0.86.zip - R64-1-1.86, Saccharomyces_cerevisiae, http://downloads.sourceforge.net/project/snpeff/databases/v4_3/snpEff_v4_3_R64-1-1.86.zip ### __Data Analysis Steps__ 1. Trimming the adapters with TrimGalore. - This step is particularly important when the reads are long and the fragments are short - resulting in sequencing adapters at the ends of reads. If adapter is not removed the read will not map. TrimGalore can recognize standard adapters, such as Illumina or Nextera/Tn5 adapters. 2. Generate quality control statistics of trimmed, unmapped sequence data 3. Run germline variant calling pipeline, custom wrapper script implementing Steps 1 - 17 of the Broad Institute's best practices gatk4 germline variant calling pipeline (https://gencore.bio.nyu.edu/variant-calling-pipeline-gatk4/) ### __References__ 1. https://gencore.bio.nyu.edu/variant-calling-pipeline-gatk4/ 2. https://gatk.broadinstitute.org/hc/en-us/articles/360035535932-Germline-short-variant-discovery-SNPs-Indels- 3. https://software.broadinstitute.org/software/igv/VCF

https://github.com/datirium/workflows.git

Path: workflows/vc-germline-pe.cwl

Branch/Commit ID: 22880e0f41d0420a17d643e8a6e8ee18165bbfbf
workflow graph Build STAR indices

Workflow runs [STAR](https://github.com/alexdobin/STAR) v2.5.3a (03/17/2017) PMID: [23104886](https://www.ncbi.nlm.nih.gov/pubmed/23104886) to build indices for reference genome provided in a single FASTA file as fasta_file input and GTF annotation file from annotation_gtf_file input. Generated indices are saved in a folder with the name that corresponds to the input genome.

https://github.com/datirium/workflows.git

Path: workflows/star-index.cwl

Branch/Commit ID: 4dcc405133f22c63478b6091fb5f591b6be8950f
workflow graph Unaligned BAM to BQSR and VCF

https://github.com/genome/analysis-workflows.git

Path: definitions/subworkflows/bam_to_bqsr_no_dup_marking.cwl

Branch/Commit ID: a23f42ef49c10a588fd35a3afaad5de03e253533
workflow graph gathered exome alignment and somatic variant detection for cle purpose

https://github.com/genome/analysis-workflows.git

Path: definitions/pipelines/somatic_exome_cle_gathered.cwl

Branch/Commit ID: 3034168d652bfa930ba09af20e473a4564a8010d
workflow graph DESeq2 Multi-factor Analysis

DESeq2 Multi-factor Analysis Runs DeSeq2 multi-factor analysis with manual control over major parameters

 https://github.com/datirium/workflows.git

Path: workflows/deseq-multi-factor.cwl

Branch/Commit ID: b4d578c2ba4713a5a22163d9f8c7105acda1f22e
workflow graph blastp_wnode_naming

https://github.com/ncbi/pgap.git

Path: task_types/tt_blastp_wnode_naming.cwl

Branch/Commit ID: 16e3915d2a357e2a861b30911c832e5ddc0c1784
workflow graph cluster_blastp_wnode and gpx_qdump combined

https://github.com/ncbi/pgap.git

Path: task_types/tt_cluster_and_qdump.cwl

Branch/Commit ID: 733ab7198a66a0153d0f03c3022ab53c17325ff8