Explore Workflows
View already parsed workflows here or click here to add your own
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heatmap-prepare.cwl
Workflow runs homer-make-tag-directory.cwl tool using scatter for the following inputs - bam_file - fragment_size - total_reads `dotproduct` is used as a `scatterMethod`, so one element will be taken from each array to construct each job: 1) bam_file[0] fragment_size[0] total_reads[0] 2) bam_file[1] fragment_size[1] total_reads[1] ... N) bam_file[N] fragment_size[N] total_reads[N] `bam_file`, `fragment_size` and `total_reads` arrays should have the identical order. |
Path: subworkflows/heatmap-prepare.cwl Branch/Commit ID: 4106b7dc96e968db291b7a61ecd1641aa3b3dd6d |
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BWA index pipeline
This workflow indexes the input reference FASTA with bwa, and generates faidx and dict file using samtools. This index sample can then be used as input into the germline variant calling workflow, or others that may include this workflow as an upstream source. ### __Inputs__ - FASTA file of the reference genome that will be indexed. ### __Outputs__ - Directory containing the original FASTA, faidx, dict, and bwa index files. - stdout log file (output in Overview tab as well) - stderr log file ### __Data Analysis Steps__ 1. cwl calls dockercontainer robertplayer/scidap-gatk4 to index reference FASTA with bwa, and generates faidx and dict files using samtools ### __References__ - Li, H., & Durbin, R. (2009). Fast and accurate short read alignment with Burrows–Wheeler transform. Bioinformatics, 25(14), 1754–1760. |
Path: workflows/bwa-index.cwl Branch/Commit ID: 12e5256de1b680c551c87fd5db6f3bc65428af67 |
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directory.cwl
Inspect provided directory and return filenames. Generate a new directory and return it (including content). |
Path: tests/wf/directory.cwl Branch/Commit ID: 280a852e74aec08cf79687e8004e17b1ab464534 |
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Bacterial Annotation, pass 1, genemark training, by HMMs (first pass)
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Path: bacterial_annot/wf_bacterial_annot_pass1.cwl Branch/Commit ID: 343cb00abda2bc06daf9a32e1386c835f324ae6e |
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Trim Galore RNA-Seq pipeline paired-end
The original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for a **pair-end** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the single-end RNA-Seq data. It performs the following steps: 1. Trim adapters from input FASTQ files 2. Use STAR to align reads from input FASTQ files according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 3. Use fastx_quality_stats to analyze input FASTQ files and generate quality statistics files 4. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ files to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
Path: workflows/trim-rnaseq-pe.cwl Branch/Commit ID: dda9e6e06a656b7b3fa7504156474b962fe3953c |
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cluster_blastp_wnode and gpx_qdump combined
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Path: task_types/tt_cluster_and_qdump.cwl Branch/Commit ID: cb15f907132fb90bc66b39bb0af3c211801feba1 |
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Subworkflow to allow calling cnvkit with cram instead of bam files
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Path: definitions/subworkflows/cram_to_cnvkit.cwl Branch/Commit ID: 3042812447d9e8889c6118986490e9c9b9b13223 |
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umi duplex alignment fastq workflow
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Path: definitions/pipelines/alignment_umi_duplex.cwl Branch/Commit ID: bfcb5ffbea3d00a38cc03595d41e53ea976d599d |
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kmer_cache_store
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Path: task_types/tt_kmer_cache_store.cwl Branch/Commit ID: e668f9c4047f1971ae53040a5af3eccc4bfc3c53 |
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Trim Galore RNA-Seq pipeline single-read
The original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for a **single-end** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the single-end RNA-Seq data. It performs the following steps: 1. Trim adapters from input FASTQ file 2. Use STAR to align reads from input FASTQ file according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 3. Use fastx_quality_stats to analyze input FASTQ file and generate quality statistics file 4. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ file to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
Path: workflows/trim-rnaseq-se.cwl Branch/Commit ID: 44214a9d02e6d85b03eb708552ed812ae3d4a733 |
