Explore Workflows
View already parsed workflows here or click here to add your own
| Graph | Name | Retrieved From | View |
|---|---|---|---|
|
|
Deprecated. Single-Cell Preprocessing Pipeline
Devel version of Single-Cell Preprocessing Pipeline =================================================== |
Path: workflows/single-cell-preprocess.cwl Branch/Commit ID: 22880e0f41d0420a17d643e8a6e8ee18165bbfbf |
|
|
|
Generate genome indices for STAR & bowtie
Creates indices for: * [STAR](https://github.com/alexdobin/STAR) v2.5.3a (03/17/2017) PMID: [23104886](https://www.ncbi.nlm.nih.gov/pubmed/23104886) * [bowtie](http://bowtie-bio.sourceforge.net/tutorial.shtml) v1.2.0 (12/30/2016) It performs the following steps: 1. `STAR --runMode genomeGenerate` to generate indices, based on [FASTA](http://zhanglab.ccmb.med.umich.edu/FASTA/) and [GTF](http://mblab.wustl.edu/GTF2.html) input files, returns results as an array of files 2. Outputs indices as [Direcotry](http://www.commonwl.org/v1.0/CommandLineTool.html#Directory) data type 3. Separates *chrNameLength.txt* file from Directory output 4. `bowtie-build` to generate indices requires genome [FASTA](http://zhanglab.ccmb.med.umich.edu/FASTA/) file as input, returns results as a group of main and secondary files |
Path: workflows/genome-indices.cwl Branch/Commit ID: 22880e0f41d0420a17d643e8a6e8ee18165bbfbf |
|
|
|
running cellranger mkfastq and count
|
Path: definitions/subworkflows/cellranger_mkfastq_and_count.cwl Branch/Commit ID: 049f4aeff4c4a1b8421cac9b1c1c1f0da5848315 |
|
|
|
count-lines8-wf.cwl
|
Path: cwltool/schemas/v1.0/v1.0/count-lines8-wf.cwl Branch/Commit ID: 26870e38cec81af880cd3e4789ae6cee8fc27020 |
|
|
|
Single-cell Assign Cell Types
Single-cell Assign Cell Types ============================= Assigns cell types to Seurat clusters. |
Path: workflows/sc-assign-cell-types.cwl Branch/Commit ID: 36fd18f11e939d3908b1eca8d2939402f7a99b0f |
|
|
|
RNA-Seq pipeline single-read strand specific
Note: should be updated The original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for **strand specific single-read** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the single-read RNA-Seq data. It performs the following steps: 1. Use STAR to align reads from input FASTQ file according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 2. Use fastx_quality_stats to analyze input FASTQ file and generate quality statistics file 3. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ file to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
Path: workflows/rnaseq-se-dutp.cwl Branch/Commit ID: 36fd18f11e939d3908b1eca8d2939402f7a99b0f |
|
|
|
Single-cell RNA-Seq Cluster Analysis
Single-cell RNA-Seq Cluster Analysis Clusters single-cell RNA-Seq datasets, identifies gene markers. |
Path: workflows/sc-rna-cluster.cwl Branch/Commit ID: 36fd18f11e939d3908b1eca8d2939402f7a99b0f |
|
|
|
Motif Finding with HOMER from FASTA files
Motif Finding with HOMER from FASTA files --------------------------------------------------- HOMER contains a novel motif discovery algorithm that was designed for regulatory element analysis in genomics applications (DNA only, no protein). It is a differential motif discovery algorithm, which means that it takes two sets of sequences and tries to identify the regulatory elements that are specifically enriched in on set relative to the other. It uses ZOOPS scoring (zero or one occurrence per sequence) coupled with the hypergeometric enrichment calculations (or binomial) to determine motif enrichment. HOMER also tries its best to account for sequenced bias in the dataset. It was designed with ChIP-Seq and promoter analysis in mind, but can be applied to pretty much any nucleic acids motif finding problem. For more information please refer to: ------------------------------------- [Official documentation](http://homer.ucsd.edu/homer/motif/) |
Path: workflows/homer-motif-analysis.cwl Branch/Commit ID: ee66d03be8a7fd61367db40c37a973ff55ece4da |
|
|
|
trim-chipseq-pe.cwl
Runs ChIP-Seq BioWardrobe basic analysis with paired-end input data files. |
Path: workflows/trim-chipseq-pe.cwl Branch/Commit ID: 801f7b363e0599b9a28ecda696dfdb1c0e40ce71 |
|
|
|
Varscan Workflow
|
Path: definitions/subworkflows/varscan_pre_and_post_processing.cwl Branch/Commit ID: c6bbd4cdd612b3b5cc6e9000df4800c21e192bf5 |
