Explore Workflows
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Motif Finding with HOMER with random background regions
Motif Finding with HOMER with random background regions --------------------------------------------------- HOMER contains a novel motif discovery algorithm that was designed for regulatory element analysis in genomics applications (DNA only, no protein). It is a differential motif discovery algorithm, which means that it takes two sets of sequences and tries to identify the regulatory elements that are specifically enriched in on set relative to the other. It uses ZOOPS scoring (zero or one occurrence per sequence) coupled with the hypergeometric enrichment calculations (or binomial) to determine motif enrichment. HOMER also tries its best to account for sequenced bias in the dataset. It was designed with ChIP-Seq and promoter analysis in mind, but can be applied to pretty much any nucleic acids motif finding problem. Here is how we generate background for Motifs Analysis ------------------------------------- 1. Take input file with regions in a form of “chr\" “start\" “end\" 2. Sort and remove duplicates from this regions file 3. Extend each region in 20Kb into both directions 4. Merge all overlapped extended regions 5. Subtract not extended regions from the extended ones 6. Randomly distribute not extended regions within the regions that we got as a result of the previous step 7. Get fasta file from these randomly distributed regions (from the previous step). Use it as background For more information please refer to: ------------------------------------- [Official documentation](http://homer.ucsd.edu/homer/motif/) |
Path: workflows/homer-motif-analysis.cwl Branch/Commit ID: c6bfa0de917efb536dd385624fc7702e6748e61d |
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count-lines5-wf.cwl
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Path: tests/count-lines5-wf.cwl Branch/Commit ID: 664835e83eb5e57eee18a04ce7b05fb9d70d77b7 |
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Run pindel on provided region
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Path: definitions/subworkflows/pindel_region.cwl Branch/Commit ID: f42c889734c8f709ad2fd9090493bcaac8326c98 |
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Workflow to run pVACseq from detect_variants and rnaseq pipeline outputs
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Path: definitions/subworkflows/pvacseq.cwl Branch/Commit ID: 735be84cdea041fcc8bd8cbe5728b29ca3586a21 |
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Single-cell ATAC-Seq Cluster Analysis
Single-cell ATAC-Seq Cluster Analysis Clusters single-cell ATAC-Seq datasets, identifies differentially accessible peaks. |
Path: workflows/sc-atac-cluster.cwl Branch/Commit ID: 22880e0f41d0420a17d643e8a6e8ee18165bbfbf |
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Cell Ranger Aggregate
Cell Ranger Aggregate Aggregates outputs from multiple runs of Cell Ranger Count Gene Expression or Cell Ranger Multi Gene Expression and V(D)J Repertoire Profiling experiments |
Path: workflows/cellranger-aggr.cwl Branch/Commit ID: 7030da528559c7106d156284e50ff0ecedab0c4e |
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trim-chipseq-pe.cwl
Runs ChIP-Seq BioWardrobe basic analysis with paired-end input data files. |
Path: workflows/trim-chipseq-pe.cwl Branch/Commit ID: ca2dbb71d0537b1d93a8bd44719250cf8949b157 |
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sum-wf.cwl
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Path: cwltool/schemas/v1.0/v1.0/sum-wf.cwl Branch/Commit ID: 75271e2a0887d47cca4077b60dd51ac763c09b63 |
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Deprecated. Single-Cell Preprocessing Pipeline
Devel version of Single-Cell Preprocessing Pipeline =================================================== |
Path: workflows/single-cell-preprocess.cwl Branch/Commit ID: 261c0232a7a40880f2480b811ed2d7e89c463869 |
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revsort.cwl
Reverse the lines in a document, then sort those lines. |
Path: cwltool/schemas/v1.0/v1.0/revsort.cwl Branch/Commit ID: 886a6ac41c685f20d39e352f9c657e59f3312265 |
