Explore Workflows
View already parsed workflows here or click here to add your own
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schemadef-wf.cwl
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Path: cwltool/schemas/v1.0/v1.0/schemadef-wf.cwl Branch/Commit ID: 1e5ad10c6b0d1c5f531737d12ef64062a00baef2 |
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sec-wf.cwl
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Path: tests/wf/sec-wf.cwl Branch/Commit ID: 1b5633876aabd4cb57ef3f1fe91c853f3ee82e46 |
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Build Bismark indices
Copy fasta_file file to the folder and run run bismark_genome_preparation script to prepare indices for Bismark Methylation Analysis. Bowtie2 aligner is used by default. The name of the output indices folder is equal to the genome input. |
Path: workflows/bismark-index.cwl Branch/Commit ID: 7030da528559c7106d156284e50ff0ecedab0c4e |
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Bismark Methylation PE
Sequence reads are first cleaned from adapters and transformed into fully bisulfite-converted forward (C->T) and reverse read (G->A conversion of the forward strand) versions, before they are aligned to similarly converted versions of the genome (also C->T and G->A converted). Sequence reads that produce a unique best alignment from the four alignment processes against the bisulfite genomes (which are running in parallel) are then compared to the normal genomic sequence and the methylation state of all cytosine positions in the read is inferred. A read is considered to align uniquely if an alignment has a unique best alignment score (as reported by the AS:i field). If a read produces several alignments with the same number of mismatches or with the same alignment score (AS:i field), a read (or a read-pair) is discarded altogether. On the next step we extract the methylation call for every single C analysed. The position of every single C will be written out to a new output file, depending on its context (CpG, CHG or CHH), whereby methylated Cs will be labelled as forward reads (+), non-methylated Cs as reverse reads (-). The output of the methylation extractor is then transformed into a bedGraph and coverage file. The bedGraph counts output is then used to generate a genome-wide cytosine report which reports the number on every single CpG (optionally every single cytosine) in the genome, irrespective of whether it was covered by any reads or not. As this type of report is informative for cytosines on both strands the output may be fairly large (~46mn CpG positions or >1.2bn total cytosine positions in the human genome). |
Path: workflows/bismark-methylation-pe.cwl Branch/Commit ID: 69643d8c15f5357a320aa7e2f6adb2e71302fd20 |
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Cell Ranger Aggregate (RNA+ATAC)
Cell Ranger Aggregate (RNA+ATAC) Combines outputs from multiple runs of “Cell Ranger Count (RNA+ATAC)” pipeline. The results of this workflow are primarily used in “Single-Cell Multiome ATAC and RNA-Seq Filtering Analysis” pipeline. |
Path: workflows/cellranger-arc-aggr.cwl Branch/Commit ID: 69643d8c15f5357a320aa7e2f6adb2e71302fd20 |
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format_rrnas_from_seq_entry
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Path: task_types/tt_format_rrnas_from_seq_entry.cwl Branch/Commit ID: 72804b6506c9f54ec75627f82aafe6a28d7a49fa |
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kmer_cache_store
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Path: task_types/tt_kmer_cache_store.cwl Branch/Commit ID: cabb1a9a95244e93294727be8cf5816c38992cb0 |
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kmer_ref_compare_wnode
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Path: task_types/tt_kmer_ref_compare_wnode.cwl Branch/Commit ID: c28cfb9882dedd3c522160f933cff1050ae24100 |
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ROSE: rank ordering of super-enhancers
Super-enhancers, consist of clusters of enhancers that are densely occupied by the master regulators and Mediator. Super-enhancers differ from typical enhancers in size, transcription factor density and content, ability to activate transcription, and sensitivity to perturbation. Use to create stitched enhancers, and to separate super-enhancers from typical enhancers using sequencing data (.bam) given a file of previously identified constituent enhancers (.gff) |
Path: workflows/super-enhancer.cwl Branch/Commit ID: 69643d8c15f5357a320aa7e2f6adb2e71302fd20 |
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trim-chipseq-se.cwl
Runs ChIP-Seq BioWardrobe basic analysis with single-end data file. |
Path: workflows/trim-chipseq-se.cwl Branch/Commit ID: de847468843203ce92b6d19323c5fe77dc488e34 |
