Explore Workflows
View already parsed workflows here or click here to add your own
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bam-bedgraph-bigwig.cwl
Workflow converts input BAM file into bigWig and bedGraph files. Input BAM file should be sorted by coordinates (required by `bam_to_bedgraph` step). If `split` input is not provided use true by default. Default logic is implemented in `valueFrom` field of `split` input inside `bam_to_bedgraph` step to avoid possible bug in cwltool with setting default values for workflow inputs. `scale` has higher priority over the `mapped_reads_number`. The last one is used to calculate `-scale` parameter for `bedtools genomecov` (step `bam_to_bedgraph`) only in a case when input `scale` is not provided. All logic is implemented inside `bedtools-genomecov.cwl`. `bigwig_filename` defines the output name only for generated bigWig file. `bedgraph_filename` defines the output name for generated bedGraph file and can influence on generated bigWig filename in case when `bigwig_filename` is not provided. All workflow inputs and outputs don't have `format` field to avoid format incompatibility errors when workflow is used as subworkflow. |
Path: tools/bam-bedgraph-bigwig.cwl Branch/Commit ID: b4d578c2ba4713a5a22163d9f8c7105acda1f22e |
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checkm_wnode
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Path: task_types/tt_checkm_wnode.cwl Branch/Commit ID: 1e16653514fd5629a704516eb447043c9fd0a53b |
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FASTQ Download
FASTQ Download Assists in downloading problematic single-cell sequencing data from Sequence Read Archive (SRA) |
Path: workflows/fastq-download.cwl Branch/Commit ID: 549fac35bf6b8b1c25af0f4f6c3f162c40dc130e |
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taxcheck.cwl
Perform taxonomic identification tasks on an input genome |
Path: taxcheck.cwl Branch/Commit ID: 1e16653514fd5629a704516eb447043c9fd0a53b |
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dicom-workflow.cwl
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Path: dicom-workflow.cwl Branch/Commit ID: f27077cc61b284b3b656615c5a53b756cd9df0d7 |
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scatter-wf4.cwl#main
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Path: cwltool/schemas/v1.0/v1.0/scatter-wf4.cwl Branch/Commit ID: fd6e054510e2bb65eed4069a3a88013d7ecbb99c Packed ID: main |
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mut2.cwl
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Path: tests/wf/mut2.cwl Branch/Commit ID: 75271e2a0887d47cca4077b60dd51ac763c09b63 |
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step_valuefrom5_wf_v1_0.cwl
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Path: testdata/step_valuefrom5_wf_v1_0.cwl Branch/Commit ID: e949503ac0dd7e22ba9b04ac51926d13780f9cee |
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Trim Galore RNA-Seq pipeline single-read strand specific
Note: should be updated The original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for a **single-end** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the single-end RNA-Seq data. It performs the following steps: 1. Trim adapters from input FASTQ file 2. Use STAR to align reads from input FASTQ file according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 3. Use fastx_quality_stats to analyze input FASTQ file and generate quality statistics file 4. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ file to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
Path: workflows/trim-rnaseq-se-dutp.cwl Branch/Commit ID: ddc35c559d1ac6aab4972fe1a2b63300c4373f54 |
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Complete Mapping and Quality Control pipeline for Paired-end data that also executes functions of the metaseqR2 package
A workflow which: i) Runs Hisat2 on each fastq pair while generating the fastq files containing unmapped reads ii) Runs Bowtie2 using the --very-sensitive-local option, on the unmapped reads iii) A subworkflow is employed to prepare BAM files, corresponding to the unmapped-remaped reads, to be merged with the mapped reads form Hisat2 iv) After the merged BAMs are created, they are sorted and assigned a user defined name (according to sample identifier) v) Corresponding index file for each sample is generated. vi) BAMs and index files are used to create bigWig files, to be used for exploring RNA signal in genome browsers vii) Above files are also used to calculate counts (counts.RData object) and generate the metaseqR2 html report viii) At the same time it performs quality control over the FASTQ using fastqc and assembles the MultiQC report |
Path: CWL/workflows/mapping-pe-qc-r.cwl Branch/Commit ID: 6a1a6b9d5a152e783fe3f794ccce35387d02fd0d |
