Explore Workflows
View already parsed workflows here or click here to add your own
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DiffBind - Differential Binding Analysis of ChIP-Seq Peak Data
Differential Binding Analysis of ChIP-Seq Peak Data --------------------------------------------------- DiffBind processes ChIP-Seq data enriched for genomic loci where specific protein/DNA binding occurs, including peak sets identified by ChIP-Seq peak callers and aligned sequence read datasets. It is designed to work with multiple peak sets simultaneously, representing different ChIP experiments (antibodies, transcription factor and/or histone marks, experimental conditions, replicates) as well as managing the results of multiple peak callers. For more information please refer to: ------------------------------------- Ross-Innes CS, Stark R, Teschendorff AE, Holmes KA, Ali HR, Dunning MJ, Brown GD, Gojis O, Ellis IO, Green AR, Ali S, Chin S, Palmieri C, Caldas C, Carroll JS (2012). “Differential oestrogen receptor binding is associated with clinical outcome in breast cancer.” Nature, 481, -4. |
Path: workflows/diffbind.cwl Branch/Commit ID: 282762f8bbaea57dd488115745ef798e128bade1 |
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scatter-wf1.cwl
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Path: cwltool/schemas/v1.0/v1.0/scatter-wf1.cwl Branch/Commit ID: c6cced7a2e6389d2eb43342e702677ccb7c7497c |
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Cell Ranger Reference (VDJ)
Cell Ranger Reference (VDJ) Builds a reference genome of a selected species for V(D)J contigs assembly and clonotype calling. The results of this workflow are used in “Cell Ranger Count (RNA+VDJ)” pipeline. |
Path: workflows/cellranger-mkvdjref.cwl Branch/Commit ID: 261c0232a7a40880f2480b811ed2d7e89c463869 |
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chipseq-gen-bigwig.cwl
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Path: subworkflows/chipseq-gen-bigwig.cwl Branch/Commit ID: d6ec0dee61ef65a110e10141bde1a79332a64ab0 |
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Index building sub-workflow
Build genome and transcriptome indexes for alignment, quantification. |
Path: amp-rnaseq_reprocessing/amp-rnaseq_reprocess-workflow/wf-buildindexes.cwl Branch/Commit ID: 50e640ce16be4287b2e87381850a211ad3e70dc1 |
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step-valuefrom2-wf.cwl
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Path: cwltool/schemas/v1.0/v1.0/step-valuefrom2-wf.cwl Branch/Commit ID: b82ce7ae901a54c7a062fd5eefd8d5ceb5a4d684 |
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RNA-Seq pipeline paired-end strand specific
The original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for a **paired-end** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the paired-end RNA-Seq data. It performs the following steps: 1. Use STAR to align reads from input FASTQ files according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 2. Use fastx_quality_stats to analyze input FASTQ files and generate quality statistics files 3. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 4. Generate BigWig file on the base of sorted BAM file 5. Map input FASTQ files to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 6. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
Path: workflows/rnaseq-pe-dutp.cwl Branch/Commit ID: 2b8146f76595f0c4d8bf692de78b21280162b1d0 |
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ROSE: rank ordering of super-enhancers
Super-enhancers, consist of clusters of enhancers that are densely occupied by the master regulators and Mediator. Super-enhancers differ from typical enhancers in size, transcription factor density and content, ability to activate transcription, and sensitivity to perturbation. Use to create stitched enhancers, and to separate super-enhancers from typical enhancers using sequencing data (.bam) given a file of previously identified constituent enhancers (.gff) |
Path: workflows/super-enhancer.cwl Branch/Commit ID: ebbf23764ede324cabc064bd50647c1f643726fa |
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gcaccess_from_list
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Path: task_types/tt_gcaccess_from_list.cwl Branch/Commit ID: 733ab7198a66a0153d0f03c3022ab53c17325ff8 |
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PCA - Principal Component Analysis
Principal Component Analysis --------------- Principal component analysis (PCA) is a statistical procedure that uses an orthogonal transformation to convert a set of observations of possibly correlated variables (entities each of which takes on various numerical values) into a set of values of linearly uncorrelated variables called principal components. The calculation is done by a singular value decomposition of the (centered and possibly scaled) data matrix, not by using eigen on the covariance matrix. This is generally the preferred method for numerical accuracy. |
Path: workflows/pca.cwl Branch/Commit ID: ebbf23764ede324cabc064bd50647c1f643726fa |
