Explore Workflows
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Trim Galore SMARTer RNA-Seq pipeline paired-end strand specific
https://chipster.csc.fi/manual/library-type-summary.html Modified original [BioWardrobe's](https://biowardrobe.com) [PubMed ID:26248465](https://www.ncbi.nlm.nih.gov/pubmed/26248465) **RNA-Seq** basic analysis for a **pair-end** experiment. A corresponded input [FASTQ](http://maq.sourceforge.net/fastq.shtml) file has to be provided. Current workflow should be used only with the single-end RNA-Seq data. It performs the following steps: 1. Trim adapters from input FASTQ files 2. Use STAR to align reads from input FASTQ files according to the predefined reference indices; generate unsorted BAM file and alignment statistics file 3. Use fastx_quality_stats to analyze input FASTQ files and generate quality statistics files 4. Use samtools sort to generate coordinate sorted BAM(+BAI) file pair from the unsorted BAM file obtained on the step 1 (after running STAR) 5. Generate BigWig file on the base of sorted BAM file 6. Map input FASTQ files to predefined rRNA reference indices using Bowtie to define the level of rRNA contamination; export resulted statistics to file 7. Calculate isoform expression level for the sorted BAM file and GTF/TAB annotation file using GEEP reads-counting utility; export results to file |
Path: workflows/trim-rnaseq-pe-smarter-dutp.cwl Branch/Commit ID: ebbf23764ede324cabc064bd50647c1f643726fa |
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step-valuefrom2-wf.cwl
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Path: tests/step-valuefrom2-wf.cwl Branch/Commit ID: 664835e83eb5e57eee18a04ce7b05fb9d70d77b7 |
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Chipseq alignment for mouse with qc and creating homer tag directory
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Path: definitions/pipelines/chipseq_alignment_mouse.cwl Branch/Commit ID: 22fce2dbdada0c4135b6f0677f78535cf980cb07 |
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scatter GATK HaplotypeCaller over intervals
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Path: detect_variants/gatk_haplotypecaller_iterator.cwl Branch/Commit ID: ee1afbcbe01fd17715b54d23e556a23086aeec57 |
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FASTQ to BQSR
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Path: definitions/subworkflows/fastq_to_bqsr.cwl Branch/Commit ID: 39ac49f5d080bbb6bfa97246f46a5b621254f622 |
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wffail.cwl
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Path: tests/wf/wffail.cwl Branch/Commit ID: dbc4c4c2ad30ed31367b4fbcc3bb4084fdcabaa2 |
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Build Bismark indices
Copy fasta_file file to the folder and run run bismark_genome_preparation script to prepare indices for Bismark Methylation Analysis. Bowtie2 aligner is used by default. The name of the output indices folder is equal to the genome input. |
Path: workflows/bismark-index.cwl Branch/Commit ID: 22880e0f41d0420a17d643e8a6e8ee18165bbfbf |
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Whole genome alignment and somatic variant detection
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Path: definitions/pipelines/somatic_wgs.cwl Branch/Commit ID: 2e0562a5c3cd7aac24af4c622a5ae68a7fb23a71 |
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sec-wf-out.cwl
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Path: tests/wf/sec-wf-out.cwl Branch/Commit ID: 63f539ba60e91f0cb3ce7cda2c5da5c65525c375 |
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umi duplex alignment fastq workflow
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Path: definitions/pipelines/alignment_umi_duplex.cwl Branch/Commit ID: 77ec4f26eb14ed82481828bd9f6ef659cfd8b40f |
